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A New Portable In Vitro Exposure Cassette for Aerosol Sampling

DOI :

10.3791/58916-v

February 22nd, 2019

February 22nd, 2019

6,959 Views

1Department of Chemical and Life Science Engineering, Virginia Commonwealth University

Here, we present a protocol to perform portable cellular aerosol exposures and measure cellular response. The method uses cells, grown at the air-liquid interface, mimicking in vivo physiology. Cellular response to copper nanoparticle aerosols was observed as oxidative stress through reactive oxygen species generation and cytotoxicity as lactate dehydrogenase release.

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