Preparation of Cell (COS1) Aggregates Genetically-modified to Secrete a Candidate Molecule in 3-D collagen Hydrogels
4:03
Generation of Embryonic Explant for Culture
5:54
Preparation of 3-D Co-cultures in Collagen Hydrogels
7:25
Results: Analysis of Axonal Growth and Behavior
8:39
Conclusion
Transcription
This method is useful to order to analyze the role of some molecules in axonal guidance and neuronal migration during neuro system development. The main advantage of this technique is that the analysis of the results is quick and simple and doesn'
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Here, we provide a method for analyzing the behavior of growing axons in 3D matrices, mimicking their natural development.