Generation of Clonal EED Knockout Mouse Embryonic Stem Cells
2:51
Engineering Cre-ERT2 Based Inducible EED Expression
4:11
Following Nucleation and Spreading of PRC2 Activity on Chromatin
5:10
Results: Validations and Representative Applications of Inducible EED Rescue Systems
6:43
Conclusion
Transcription
We outline a system to assess the dynamic formation of chromatin domains. We utilize this system to follow the recruitment and spreading of PRC2-mediated chromatin domains in cells. The process can be paused at any time to analyze the events in pr
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This method is designed to follow formation of PRC2-mediated chromatin domains in cell lines, and the method can be adapted to many other systems.