Results: Representative LHVS Inhibition Efficacy Against Intracellular Toxoplasma Growth
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Conclusion
Transcription
These sensitive strategies can be used to evaluate the inhibition efficacy of chemical inhibitors and in combination with CRISPR-Cas9 gene deletion to investigate the drug targets. Instead of using an endpoint assay, parasite growth can be monitor
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Presented here is a protocol to evaluate the inhibition efficacy of chemical compounds against in vitro intracellular growth of Toxoplasma gondii using a luciferase-based growth assay. The technique is used to confirm inhibition specificity by genetic deletion of the corresponding target gene. The inhibition of LHVS against TgCPL protease is evaluated as an example.