Ischemic stroke is a clinical condition characterized by hypoperfusion of brain tissue and results in neuronal loss. Numerous evidences suggest that the interaction between glia and urinal cells exert the pressure effects after an ischemic event.
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Here, we present a simple approach using specific culture media that allows the establishment of neuron- and astrocyte-enriched cultures, or neuron-glia cultures from the embryonic cortex, with high yield and reproducibility.