November 3rd, 2020
•Mouse models allow studying key mechanisms of arrhythmogenesis. For this purpose, high quality cardiomyocytes are necessary to perform patch-clamp measurements. Here, a method to isolate murine atrial and ventricular myocytes via retrograde enzyme-based Langendorff perfusion, which allows simultaneous measurements of calcium-transients and L-type calcium current, is described.
Vidéos Connexes
Isolation and Genetic Manipulation of Adult Cardiac Myocytes for Confocal Imaging Video (Video) | JoVE
Isolation and Kv Channel Recordings in Murine Atrial and Ventricular Cardiomyocytes Video (Video) | JoVE
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents Video (Video) | JoVE
Preparation of Pancreatic Acinar Cells for the Purpose of Calcium Imaging, Cell Injury Measurements, and Adenoviral Infection Video (Video) | JoVE
Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry Video (Video) | JoVE
Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer Video (Video) | JoVE
Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements Video (Video) | JoVE
Development and Characterization of In Vitro Microvessel Network and Quantitative Measurements of Endothelial [Ca2+]i and Nitric Oxide Production Video (Video) | JoVE
Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy Video (Video) | JoVE
Simultaneous Isolation of High Quality Cardiomyocytes, Endothelial Cells, and Fibroblasts from an Adult Rat Heart Video (Video) | JoVE