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Targeting the Corticospinal Tract in Neonatal Rats with a Double-Viral Vector using Combined Brain and Spine Surgery

DOI :

10.3791/62698-v

June 30th, 2021

June 30th, 2021

1,765 Views

1Shriners Hospitals Pediatric Research Center, Lewis Katz School of Medicine, Temple University

This protocol demonstrates a novel method for applying gene therapies to subpopulations of cells in neonatal rats at postnatal ages 5-10 days by injecting an anterograde chemogenetic modifier into the somatomotor cortex and a retrogradely transportable Cre recombinase into the cervical spinal cord.

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Keywords Corticospinal Tract

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