Defining the Stopped-Flow Mixing Protocol in the Program Script
3:43
Defining Small-Angle Neutron Scattering (SANS) Parameters in the Instrument Control GUI
4:05
Collecting Standard Scattering Measurements for Data Reduction Before Beginning the Stopped-Flow Experiment to Process the Time Resolved-SANS Data
4:47
Beginning the Stopped-Flow Experiment
5:29
Removing All Backgrounds, Correcting for Detector Sensitivity, Correcting for Sample Transmission,and Analyzing the Time Resolved-SANS Data
6:08
Results: Raw Neutron Data and Scattered Intensities of Catalyzed Exchange of Outer Layer of Lipid Vesicles at Various Temperatures
7:21
Conclusion
Transcription
Stop flow small angle scattering or stop flow SANS allows us to study how nanoscale materials evolve on set timescales of seconds to minutes. The contrast between hydrogen and deuterium unique to neutron scattering makes this technique particularl
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
This protocol presents the use of a stopped-flow sample environment to quickly mix multiple liquid solutions in situ during a small-angle neutron scattering measurement and to study kinetic processes on nanometer length scales and second time scales.