Optimization of Scanning Confocal Microscope Settings
1:58
Live Imaging of the Mitochondrial Redox Response to N-methyl-D-aspartate (NMDA) Treatment
3:08
Data Analysis
5:40
Results: Effects of N-methyl-D-aspartate (NMDA) on Neuronal Mitochondria Oxidation
7:04
Conclusion
Transcription
This method allows to investigate how mitochondrial redox state is affected in pathophysiological conditions. It can also be used to assess the efficacy of treatment strategies that aim at protecting mitochondria. The main advantage of this techni
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This article describes a protocol to determine differences in basal redox state and redox responses to acute perturbations in primary hippocampal and cortical neurons using confocal live microscopy. The protocol can be applied to other cell types and microscopes with minimal modifications.