Results: Development of RYR1-Knock Out Human Muscle Cell Line
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Conclusion
Transcription
This protocol allows the development of muscle cell knock-out in any gene, in order to further study the function of this gene in muscle. The advantage is that it's cheap and faster than the development of knock-out animals to study any gene. This
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The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.