Results: Physical and Molecular Attributes of Cardiac Cells
5:07
Conclusion
Transcription
Our protocol ensures both the cell viability and isolation efficiency of single cell suspension at relatively low costs. The relatively low costs and requirements for laboratory equipment are the main advantages of this technique. Single cell sequ
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
Here, we describe a protocol to isolate a sufficient amount of single non-cardiomyocytes with high viability from a post-myocardial infarction (MI) mouse heart. This can be used for subsequent single-cell sequencing, flow cytometry analysis, and primary cell culture.