In this protocol, urethral reconstruction using a buccal mucosa graft is developed for the first time in a local pathophysiological environment, which is crucial to mimic disease. This technique allow us to develop innovative therapies and to stud
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In the present protocol, a urethral stricture induction was developed in Wistar rats, followed by urethral reconstruction with a buccal mucosa graft. A retrograde urethrogram and laser Doppler assessment were performed, validating urethral reconstruction (after stricture formation) and graft placement.