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Consistent Delivery of Adeno-Associated Virus via Lateral Tail-Vein Injection in Adult Mice

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05:03 min

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August 23rd, 2024

DOI :

10.3791/66934-v

August 23rd, 2024

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Fady Guirguis¹^,², Véronique Bolduc¹, Matthew J. Slarve³, Haiyan Zhou⁴^,⁵, Francesco Muntoni²^,⁴, Carsten G. Bönnemann¹

¹Neuromuscular and Neurogenetic Disorders of Childhood Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, ²The Dubowitz Neuromuscular Centre, Molecular Neurosciences Section, Developmental Neurosciences Research and Teaching Department, Great Ormond Street Institute of Child Health, University College London, ³Department of Molecular Microbiology and Immunology, University of Southern California, ⁴NIHR Great Ormond Street Hospital Biomedical Research Centre, ⁵Genetics and Genomic Medicine Research and Teaching Department, Great Ormond Street Institute of Child Health, University College London

Summary

Here we detail an optimized protocol for mouse lateral tail-vein injection to systemically administer adeno-associated virus (AAV) in adult mice. Additionally, we describe protocols of commonly used assays to assess AAV transduction.

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Adeno Associated Virus AAV

Muscular Dystrophies

Neuromuscular Disorders

Non viral Vectors

Lateral Tail Vein Injection

Intravenous Administration

Systemic Delivery

Preclinical Studies

Histological Detection

Fluorescent Reporter Proteins

Biodistribution

Transduction Quantification

Chapters in this video

0:00

Introduction

1:01

Lateral Tail‐Vein Injection for Systemic Therapeutic Delivery of Adeno‐Associated Virus in Mice

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