מקור / מספר מימון לביולוגיה מולקולרית של הלם Hemorrhagic GM053789

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L., Uchida, T., Kramer, G. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fluid+compartment+in+hemorrhaged+rats+after+hyperosmotic+crystalloid+and+hyperoncotic+crystalloid+resuscitation.">Fluid compartment in hemorrhaged rats after hyperosmotic crystalloid and hyperoncotic crystalloid resuscitation.</a> Am J Physiol. 270, F1-F8 (1996).</li><li>Perel, P., Roberts, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colloids+vs+crystalloids+for+fluid+resuscitation+in+critically+ill+patients.">Colloids vs crystalloids for fluid resuscitation in critically ill patients.</a> Cochrane Database System Rev. 4, CD000567-CD000567 (2007).</li></ol>

ניסויים בבעלי חיים היו בהשגחת ובוצעו בהתאם להנחיות והתקנות שנקבעו על ידי טיפול בבעלי חיים מוסדיים ועדת שימוש ההתנהגות מחקר משרד היענות של אוניברסיטת פיטסבורג, AALAS מוכר מלא / מוסד AALAC. מקורות בעלי חיים כוללים ג&#39;קסון מעבדות מעבדות צ&#39;ארלס ריברס. כל החיות לעבור הבטחה בריאות מקיף בכל דרך הספק, כמו גם את אוניברסיטת פיטסבורג בריאות הפנימיים של תוכניות חיים ניטור. מחקר זה מתבצע על פי עקרונות הממשל האמריקאי לשימוש של בעלי חיים בעלי חוליות. התוכנית רשום עם משרד החקלאות, ויש לו מכתב עם הבטחה של משרד השירות לבריאות הציבור של צער בעלי חיים במעבדה.

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><th> מכשיר </th><th> יצרן </th><th> מספר קטלוגי </th></tr><tr><td> Dumonts (SS/45 ° זווית 0.2x0.12mm-טיפ, אורך 13.5cm) </td><td> המדע כלים פיין </td><td> 11203-25 </td></tr><tr><td> מספריים כירורגיות (ישר 12cm) </td><td> המדע כלים פיין </td><td> 14068-12 </td></tr><tr><td> Hemostats (מעוקל-12.5cm) </td><td> המדע כלים פיין </td><td> 13009-12 </td></tr><tr><td> Microscissors (באביב מספריים ישר 8cm) </td><td> המדע כלים פיין </td><td> 15000-00 </td></tr><tr><td> מלקחיים (0.8mm-טיפ, 10 ס&quot;מ מעוקל-) </td><td> המדע כלים פיין </td><td> 11050-10 </td></tr><tr><td> תפר גליל 6-0 </td><td> המדע כלים פיין </td><td> 18020-60 </td></tr><tr><td> תפר 4-0 PDSII </td><td> פן Vet </td><td> ETHZ304H </td></tr><tr><td> גזה 4x4 </td><td> ניתן לרכוש דרך כל ספק גלובלי </td><td></td></tr><tr><td> כותנה קצה המוליך </td><td> ניתן לרכוש דרך כל ספק גלובלי </td><td></td></tr><tr><td> 30G מחט </td><td> ניתן לרכוש דרך כל ספק גלובלי </td><td></td></tr><tr><td> 23G מחט </td><td> ניתן לרכוש דרך כל ספק גלובלי </td><td></td></tr><tr><td> 3cc מזרק </td><td> ניתן לרכוש דרך כל ספק גלובלי </td><td></td></tr><tr><td> 5cc מזרק </td><td> ניתן לרכוש דרך כל ספק גלובלי </td><td></td></tr><tr><td> 10cc מזרק </td><td> ניתן לרכוש דרך כל ספק גלובלי </td><td></td></tr><tr><td> צינור חרוטי 50cc </td><td> ניתן לרכוש דרך כל ספק גלובלי </td><td></td></tr><tr><td> מזרק 1cc w / 25 גרם מחט </td><td> פישר סיינטיפיק </td><td> 14-826-88 </td></tr><tr><td> 10 צינורות פוליאתילן 100 `(PE-10) </td><td> פישר סיינטיפיק </td><td> 14-170-12P </td></tr><tr><td> 50 צינורות פוליאתילן 100 `(PE-50) </td><td> פישר סיינטיפיק </td><td> 14-170-12B </td></tr><tr><td> 3-דרך שסתום </td><td> פישר סיינטיפיק </td><td> NC9779127 </td></tr><tr><td> כרית כחולה כירורגי </td><td> פישר סיינטיפיק </td><td> 50-7105 </td></tr><tr><td> שדה תחבושות סטריליות </td><td> פישר סיינטיפיק </td><td> NC9517505 </td></tr><tr><td> קלטת לחמניות 1 &quot; </td><td> חברות אקספרס </td><td> MMM26001 </td></tr><tr><td> ישר רחב בצד הפה קנקנים (משמש עבור כובע קונוס האף) </td><td> VWR </td><td> 159000-058 </td></tr><tr><td> חלד מגש פלדה 8 &quot;x 11&quot; </td><td> VWR </td><td> 62687-049 </td></tr><tr><td> בין גברים לנעול Leur 3-way </td><td> VWR </td><td> 20068-909 </td></tr><tr><td> עיקור כיס 3 &quot;x8&quot; </td><td> VWR </td><td> 24008 </td></tr><tr><td> עיקור כיס 5 &quot;x10&quot; </td><td> VWR </td><td> 24010 </td></tr><tr><td> Wild M650 w / בום מיקרוסקופ לעמוד </td><td> לייקה </td><td></td></tr><tr><td> לייקה IC D מצלמה דיגיטלית / תמונה חיה </td><td> לייקה </td><td></td></tr><tr><td> Digi-Med BPA-400 &amp; מנתח מערכות אינטגרטור </td><td> Micro-Med </td><td> SYS-400 </td></tr><tr><td> TXD-310 (Digi-Med מתמר) </td><td> Micro-Med </td><td> TXD-300 </td></tr><tr><td> מחשב </td><td> Dell </td><td></td></tr><tr><td> מעקר חם חרוז מכשיר </td><td> VWR </td><td> 18000-45 </td></tr><tr><td> אוסטר A5 קוצץ W. 40 גודל להב </td><td> VWR </td><td> 10749-020 </td></tr><tr><td> במחזור לחמםגרם פנקס 18x26 </td><td> הרווארד </td><td> py872-5272 </td></tr><tr><td> מדחום רקטלי </td><td> קנט מדעי </td><td> RET-3 </td></tr></tbody></table><table border="1" style=";text-align:right;direction:rtl"><tbody><tr><th> מגיב </th><th> ריכוז </th><th> יצרן </th><th> מספר קטלוגי </th></tr><tr><td> Pentobarbital נתרן (סודיום פתרון Nembutol) </td><td> 70mg/kg </td><td> מחיאות כפים </td><td></td></tr><tr><td> עצירות HCl </td><td> 0.1mg/kg </td><td> מעבדות בדפורד </td><td></td></tr><tr><td> Lactated האצבעות הזרקת 250cc IV שקית </td><td> בקסטר </td><td></td><td></td></tr><tr><td> Aerrane (Isoflurane) </td><td> 99.9% </td><td> NLS לבריאות בעלי חיים </td><td> 105996 </td></tr><tr><td> הפרין סודיום </td><td> 1000U </td><td> Abraxis </td><td></td></tr><tr><td> נתרן כלוריד bacteriostatic </td><td> 0.9% </td><td> Hospira </td><td></td></tr><tr><td> אתיל אלכוהול </td><td> 70% </td><td> Pharmaco-AAPER </td><td></td></tr><tr><td> Triadine Povidone יוד (בבטאדין) </td><td></td><td> שלישיה disposables </td><td></td></tr></tbody></table>

fixed volume or fixed pressure: a murine model of hemorrhagic shock

זה ידוע כי אובדן דם חמור פציעה טראומטית יכולה להוביל מפל של אירועים איתות מזיקה לעתים קרובות וכתוצאה מכך התמותה. 1, 2, 3, 4, 5 אירועים אלה איתות יכול גם להוביל אלח דם ו / או תפקוד לקוי של איברים מרובים (MOD ). 6, 7, 8, 9 זה קריטי, אז כדי לחקור את הסיבות תפקוד מערכת החיסון מדוכאת ומפלים איתות מזיקה על מנת לפתח דרכים יעילות יותר כדי לעזור לחולים הסובלים מפגיעות טראומטיות. 10 זה בלחץ קבוע Hemorrhagic הלם (HS) ההליך, אם כי מאתגר מבחינה טכנית, הוא משאב מצוין עבור חקירה של תנאים אלו pathophysiologic. 11, 12, 13 ההתקדמות ההערכה של מערכות ביולוגיות, כלומר מערכות ביולוגיה אפשרו לקהילה המדעית כדי לקדם להבין רשתות פיזיולוגי מורכב דפוסי תקשורת סלולרית. 14 הלם Hemorrhagic הוכיחה להיות כלי חיוני עבור חשיפת דפוסים אלה תקשורת סלולרית ככל שהם מתייחסים תפקוד מערכת החיסון. 15, 16, 17, 18 הליך זה יכול להיות שולט! הליך זה יכול גם לשמש גם נפח קבוע או הגישה בלחץ קבוע. התאמנו את הטכניקה הזו במודל Murine כדי לשפר את תפקוד מערכת החיסון מחקר מולד אדפטיבית. 19, 20, 21 HS בשל גודלם הקטן בעכברים מציבה אתגרים ייחודיים. עקב זאת לצלילי רבים העכבר זמין, מין זה מהווה משאב שאין כמוהו לחקר התגובות ביולוגיים. המודל HS הוא מודל חשוב ללימוד דפוסי תקשורת סלולרית והתגובות של מערכות כגון מערכות המתווך הורמונליים דלקתיות, אותות סכנה, כלומר לחה upregulation PAMP כפי שהוא מעורר תגובות שונות השונים וצורות אחרות של הלם. 22, 23 , 24, 25 פיתוח זנים Murine מהונדס לבין אינדוקציה של סוכנים ביולוגיים לעכב איתות ספציפיים הציגו הזדמנויות ערך נוסף להבהיר את הבנתנו את מטה רגולציה של הולכת אותות לאחר איבוד דם חמור, כלומר HS וטראומה 26, 27 , 28, 29, 30. ישנן שיטות החייאה רבים (R) בשיתוף עם HS וטראומה. 31, 32, 33, 34 נפח קבוע החייאה שיטת פתרון הצלצול lactated בלבד (LR), שווה שלוש פעמים נפח הדם לשפוך, משמש מודל זה ללמוד מנגנונים אנדוגניים כגון פגיעה באיבר מרוחק דלקת מערכתית. 35, 36, 38 שיטה זו של החייאה הוא הוכחו כיעילים להערכת ההשפעות של HS וטראומה 38, 39.

Watch this Scientific Journal Video about Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock at JoVE.com

Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock

המודל הלם Hemorrhagic כבר משאב אמין לשחזור להקל על זיהוי והבנה של מפלי איתות הקשורים לדלקת קצה איבר נזק לאחר טראומה. מאמר זה מספק תיאור צעד אחר צעד של היבטים כירורגית מכניים הקשורים בהליך ניסיוני Hemorrhagic הלם בעכברים.

נפח קבוע או לחץ קבוע: מודל Murine של הלם Hemorrhagic

It is common knowledge that severe blood loss and traumatic injury can lead to a cascade of detrimental signaling events often resulting in mortality. 1, ...

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JoVE Journal

Medicine

24.7K Views.  University of Pittsburgh. המודל הלם Hemorrhagic כבר משאב אמין לשחזור להקל על זיהוי והבנה של מפלי איתות הקשורים לדלקת קצה איבר נזק לאחר טראומה. מאמר זה מספק תיאור צעד אחר צעד של היבטים כירורגית מכניים הקשורים בהליך ניסיוני Hemorrhagic הלם בעכברים.

Video: Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock

Murine Model of Wound Healing

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. Impaired wound healing, which occurs in diabetic patients for example, can lead to severe unfavorable outcomes such as amputation. There is, therefore, an increasing impetus to develop novel agents that promote wound repair. The testing of these has been limited to large animal models such as swine, which are often impractical. Mice represent the ideal preclinical model, as they are economical and amenable to genetic manipulation, which allows for mechanistic investigation. However, wound healing in a mouse is fundamentally different to that of humans as it primarily occurs via contraction. Our murine model overcomes this by incorporating a splint around the wound. By splinting the wound, the repair process is then dependent on epithelialization, cellular proliferation and angiogenesis, which closely mirror the biological processes of human wound healing. Whilst requiring consistency and care, this murine model does not involve complicated surgical techniques and allows for the robust testing of promising agents that may, for example, promote angiogenesis or inhibit inflammation. Furthermore, each mouse acts as its own control as two wounds are prepared, enabling the application of both the test compound and the vehicle control on the same animal. In conclusion, we demonstrate a practical, easy-to-learn, and robust model of wound healing, which is comparable to that of humans.

A murine model of cutaneous wound healing that can be used to assess therapeutic compounds in physiological and pathophysiological settings.

מודל עכברי של ריפוי פצע

Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. ...

Measurement of the Pressure-volume Curve in Mouse Lungs

In recent decades the mouse has become the primary animal model of a variety of lung diseases. In models of emphysema or fibrosis, the essential phenotypic changes are best assessed by measurement of the changes in lung elasticity. To best understand specific mechanisms underlying such pathologies in mice, it is essential to make functional measurements that can reflect the developing pathology. Although there are many ways to measure elasticity, the classical method is that of the total lung pressure-volume (PV) curve done over the whole range of lung volumes. This measurement has been made on adult lungs from nearly all mammalian species dating back almost 100 years, and such PV curves also played a major role in the discovery and understanding of the function of pulmonary surfactant in fetal lung development. Unfortunately, such total PV curves have not been widely reported in the mouse, despite the fact that they can provide useful information on the macroscopic effects of structural changes in the lung. Although partial PV curves measuring just the changes in lung volume are sometimes reported, without a measure of absolute volume, the nonlinear nature of the total PV curve makes these partial ones very difficult to interpret. In the present study, we describe a standardized way to measure the total PV curve. We have then tested the ability of these curves to detect changes in mouse lung structure in two common lung pathologies, emphysema and fibrosis. Results showed significant changes in several variables consistent with expected structural changes with these pathologies. This measurement of the lung PV curve in mice thus provides a straightforward means to monitor the progression of the pathophysiologic changes over time and the potential effect of therapeutic procedures.

Here we present a protocol to simply and reliably measure the lung pressure-volume curve in mice, showing that it is sufficiently sensitive to detect phenotypic parenchymal changes in two common lung pathologies, pulmonary fibrosis and emphysema. This metric provides a means to quantify the lung&#8217;s structural changes with developing pathology.

מדידה של עקומת לחץ-נפח ריאות עכבר

In recent decades the mouse has become the primary animal model of a variety of lung diseases. In models of emphysema or fibrosis, the essential ...

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model

Myopic individuals, especially those with severe myopia, are at higher-than-normal risk of cataract, glaucoma, retinal detachment and chorioretinal abnormalities. In addition, pathological myopia is a common irreversible cause of visual impairment and blindness1-3. Our study demonstrates the effect of scleral crosslinking using riboflavin and ultraviolet-A radiation on the development of axial myopia in a rabbit model. The axial length of the eyeball was measured by A-scan ultrasound in New Zealand white rabbits aged 13 days (male and female). The eye then underwent 360&#176; conjunctival peritomy with scleral crosslinking, followed by tarsorrhaphy. Axial elongation was induced in 13 day-old New Zealand rabbits by suturing their right eye eyelids (tarsorrhaphy). The eyes were divided into quadrants, and every quadrant had two scleral irradiation zones, each with an area of 0.2 cm&#178; and a radius of 4 mm. Crosslinking was performed by dropping 0.1% dextran-free riboflavin-5-phosphate onto the irradiation zones 20 sec before ultraviolet-A irradiation and every 20 sec during the 200 sec irradiation time. UVA radiation (370 nm) was applied perpendicular to the sclera at 57 mW/cm&#178; (total UVA light dose, 57 J/cm&#178;). Tarsorrhaphies were removed on day 55, followed by repeated axial length measurements. This study demonstrates that scleral crosslinking with riboflavin and ultraviolet-A radiation effectively prevents occlusion-induced axial elongation in a rabbit model.

We demonstrate the effect of scleral crosslinking with riboflavin and UVA on an axial elongation rabbit eye. Axial elongation was induced in 13 day-old New Zealand rabbits (male and female) by suturing their right eye eyelids (tarsorrhaphy).

Scleral Cross-linking שימוש ריבופלבין ו-סגול-קרינה למניעת Axial קוצר ראיה במודל הארנב

Myopic individuals, especially those with severe myopia, are at higher-than-normal risk of cataract, glaucoma, retinal detachment and chorioretinal ...

Uncontrolled Hemorrhagic Shock Modeled via Liver Laceration in Mice with Real Time Hemodynamic Monitoring

Uncontrolled hemorrhage is an important cause of preventable deaths among trauma patients. We have developed a murine model of uncontrolled hemorrhage via a liver laceration that results in consistent blood loss, hemodynamic alterations, and survival.
Mice undergo a standardized resection of the left-middle lobe of the liver. They are allowed to bleed without mechanical intervention. Hemostatic agents can be administered as pre-treatment or rescue therapy depending on the interest of the investigator. During the time of hemorrhage, real-time hemodynamic monitoring via a left femoral arterial line is performed. Mice are then sacrificed, blood loss is quantified, blood is collected for further analysis, and organs are harvested for analysis of injury. Experimental design is described to allow for simultaneous testing of multiple animals.
Liver hemorrhage as a model of uncontrolled hemorrhage exists in the literature, primarily in rat and porcine models. Some of these models utilize hemodynamic monitoring or quantify blood loss but lack consistency. The present model incorporates quantification of blood loss, real-time hemodynamic monitoring in a murine model that offers the advantage of using transgenic lines and a high-throughput mechanism to further investigate the pathophysiologic mechanisms in uncontrolled hemorrhage.

Uncontrolled hemorrhage, an important cause of mortality among trauma patients, can be modeled using a standard liver laceration in a murine model. This model results in consistent blood loss, survival, and allows for testing hemostatic agents. This article provides the step-by-step process to perform this valuable model.

הלם Hemorrhagic בלתי מבוקר מעוצב באמצעות laceration הכבד בעכברים עם ניטור ההודינמי בזמן אמת

Uncontrolled hemorrhage is an important cause of preventable deaths among trauma patients. We have developed a murine model of uncontrolled hemorrhage via ...

Surgical Training for the Implantation of Neocortical Microelectrode Arrays Using a Formaldehyde-fixed Human Cadaver Model

This protocol describes a procedure to assist surgeons in training for the implantation of microelectrode arrays into the neocortex of the human brain. Recent technological progress has enabled the fabrication of microelectrode arrays that allow recording the activity of multiple individual neurons in the neocortex of the human brain. These arrays have the potential to bring unique insight onto the neuronal correlates of cerebral function in health and disease. Furthermore, the identification and decoding of volitional neuronal activity opens the possibility to establish brain-computer interfaces, and thus might help restore lost neurological functions. The implantation of neocortical microelectrode arrays is an invasive procedure requiring a supra-centimetric craniotomy and the exposure of the cortical surface; thus, the procedure must be performed by an adequately trained neurosurgeon. In order to provide an opportunity for surgical training, we designed a procedure based on a human cadaver model. The use of a formaldehyde-fixed human cadaver bypasses the practical, ethical and financial difficulties of surgical practice on animals (especially non-human primates) while preserving the macroscopic structure of the head, skull, meninges and cerebral surface and allowing realistic, operating room-like positioning and instrumentation. Furthermore, the use of a human cadaver is closer to clinical daily practice than any non-human model. The major drawbacks of the cadaveric simulation are the absence of cerebral pulsation and of blood and cerebrospinal fluid circulation. We suggest that a formaldehyde-fixed human cadaver model is an adequate, practical and cost-effective approach to ensure proper surgical training before implanting microelectrode arrays in the living human neocortex.

We designed a procedure in which a formaldehyde-fixed human cadaver is used to assist neurosurgeons in training for the implantation of microelectrode arrays into the neocortex of the human brain.

אימוני ניתוח ההשתלה של מערכים Neocortical Microelectrode באמצעות מודל של פורמלדהיד-קבוע גווייה אנושי

This protocol describes a procedure to assist surgeons in training for the implantation of microelectrode arrays into the neocortex of the human brain. ...

Real-time Pressure-volume Analysis of Acute Myocardial Infarction in Mice

Acute myocardial infarction can lead to acute heart failure and cardiogenic shock. The evaluation of hemodynamics is critical for the evaluation of any potential therapeutic approach directed against acute left ventricular (LV) dysfunction. Current imaging modalities (e.g., echocardiography and magnetic resonance imaging) have several limitations since data on LV pressure cannot directly be measured. LV catheterization in mice undergoing coronary artery occlusion could serve as a novel method for a real-time evaluation of LV function.
At the beginning of the procedure, mice were anesthetized followed by endotracheal intubation. For LV catheterization, the right carotid artery was exposed via middle-neck incision. The catheter was introduced and placed into the LV cavity. Left thoracotomy was conducted and the left main coronary artery (LCA) was ligated. To induce reperfusion, the suture was released after 45 min. Pressure-volume data was recorded at all times.
Ligation of the LCA caused a decrease in LV systolic function as evidenced by a 30% reduction in stroke volume, LV ejection fraction (EF), and cardiac output. Maximum dP/dt as a parameter for LV contractility was also significantly reduced and diastolic function was severely impaired (minimum dP/dt -40%). Reperfusion over a period of 20 min did not lead to a complete recovery of LV function.
Real-time pressure-volume analysis served as a valid procedure for monitoring cardiac function during acute myocardial infarction in mice. Maintaining stable anesthesia and a standardized surgical approach was crucial to ensure valid results. As the early phase of acute myocardial infarction is critical for morbidity and mortality, the delineated method could be beneficial for preclinical evaluation of new strategies for cardioprotection.

Acute myocardial infarction in mice induces acute but incompletely characterized changes in left ventricular (LV) function. LV catheterization in mice undergoing coronary artery occlusion serves as a novel method for a real-time evaluation of LV function.

ניתוח בזמן אמת לחץ-נפח של אוטם אקוטי בעכברים

Acute myocardial infarction can lead to acute heart failure and cardiogenic shock. The evaluation of hemodynamics is critical for the evaluation of any ...

Complete and Partial Aortic Occlusion for the Treatment of Hemorrhagic Shock in Swine

Hemorrhage remains the leading cause of preventable deaths in trauma. Endovascular management of non-compressible torso hemorrhage has been at the forefront of trauma care in recent years. Since complete aortic occlusion presents serious concerns, the concept of partial aortic occlusion has gained a growing attention. Here, we present a large animal model of hemorrhagic shock to investigate the effects of a novel partial aortic balloon occlusion catheter and compare it with a catheter that works on the principles of complete aortic occlusion. Swine are anesthetized and instrumented in order to conduct controlled fixed-volume hemorrhage, and hemodynamic and physiological parameters are monitored. Following hemorrhage, aortic balloon occlusion catheters are inserted and inflated in the supraceliac aorta for 60 min, during which the animals receive whole-blood resuscitation as 20% of the total blood volume (TBV). Following balloon deflation, the animals are monitored in a critical care setting for 4 h, during which they receive fluid resuscitation and vasopressors as needed. The partial aortic balloon occlusion demonstrated improved distal mean arterial pressures (MAPs) during the balloon inflation, decreased markers of ischemia, and decreased fluid resuscitation and vasopressor use. As swine physiology and homeostatic responses following hemorrhage have been well-documented and are like those in humans, a swine hemorrhagic shock model can be used to test various treatment strategies. In addition to treating hemorrhage, aortic balloon occlusion catheters have become popular for their role in cardiac arrest, cardiac and vascular surgery, and other high-risk elective surgical procedures.

Here, we present a protocol demonstrating a hemorrhagic shock model in swine that uses aortic occlusion as a bridge to definitive care in trauma. This model has application in testing a wide range of surgical and pharmacological therapeutic strategies.

סגר אבי העורקים מלאה או חלקית לטיפול של הלם Hemorrhagic בחזיר

Hemorrhage remains the leading cause of preventable deaths in trauma. Endovascular management of non-compressible torso hemorrhage has been at the ...

Standardized Hemorrhagic Shock Induction Guided by Cerebral Oximetry and Extended Hemodynamic Monitoring in Pigs

Hemorrhagic shock ranks among the main reasons for severe injury-related death. The loss of circulatory volume and oxygen carriers can lead to an insufficient oxygen supply and irreversible organ failure. The brain exerts only limited compensation capacities and is particularly at high risk of severe hypoxic damage.This article demonstrates the reproducible induction of life-threatening hemorrhagic shock in a porcine model by means of calculated blood withdrawal. We titrate shock induction guided by near-infrared spectroscopy&#160;and extended hemodynamic monitoring to display systemic circulatory failure, as well as cerebral microcirculatory oxygen depletion. In comparison to similar models that primarily focus on predefined removal volumes for shock induction, this approach highlights a titration by means of the resulting failure of macro- and microcirculation.

Hemorrhagic shock is a severe complication in seriously injured patients, which leads to life-threatening oxygen undersupply. We present a standardized method to induce hemorrhagic shock via blood withdrawal in pigs that is guided by hemodynamics and microcirculatory cerebral oxygenation.

אינדוקציה הלם מדמם מתוקננת מונחה על ידי שחמצן מוחין וניטור הומודינמיקה מורחבת בחזירים

Hemorrhagic shock ranks among the main reasons for severe injury-related death. The loss of circulatory volume and oxygen carriers can lead to an ...

The Lower Body Positive Pressure Treadmill for Knee Osteoarthritis Rehabilitation

Here, based on a clinician&rsquo;s point-of-view, we propose a two-model lower body positive pressure (LBPP) protocol (walking and squatting models) in addition to a clinical, functional assessment methodology, including details for further encouragement of the development of non-drug surgical intervention strategies in knee osteoarthritis patients. However, we only present the effect of LBPP training in improvement of pain and knee function in one patient through three-dimensional gait analysis. The exact, long-term effects of this approach should be explored in future studies.

Here, based on a clinician&#8217;s point-of-view, we propose a two-model lower body positive pressure (LBPP) protocol (walking and squatting models) in addition to a clinical, functional assessment methodology, including details for further encouragement of the development of non-drug surgical intervention strategies in knee osteoarthritis patients. However, we only present the effect of LBPP training in improvement of pain and knee function in one patient through three-dimensional gait analysis. The exact, long-term effects of this approach should be explored in future studies.

הגוף התחתון חיובי לחץ הליכון עבור שיקום אוסטיאוארתריטיס ברך

Here, based on a clinician&rsquo;s point-of-view, we propose a two-model lower body positive pressure (LBPP) protocol (walking and squatting models) in ...

Closed Chest Biventricular Pressure-Volume Loop Recordings with Admittance Catheters in a Porcine Model

Pressure-volume (PV) loop recording enables the state-of-the-art investigation of load-independent variables of ventricular performance. Uni-ventricular evaluation is often performed in preclinical research. However, the right and left ventricles exert functional interdependence due to their parallel and serial connections, encouraging simultaneous evaluation of both ventricles. Furthermore, various pharmacological interventions may affect the ventricles and their preloads and afterloads differently. 
We describe our closed chest approach to admittance-based bi-ventricular PV loop recordings in a porcine model of acute right ventricular (RV) overload. We utilize minimally invasive techniques with all vascular accesses guided by ultrasound. PV catheters are positioned, under fluoroscopic guidance, to avoid thoracotomy in animals, as the closed chest approach maintains the relevant cardiopulmonary physiology. The admittance technology provides real-time PV loop recordings without the need for post-hoc processing. Furthermore, we explain some essential troubleshooting steps during critical timepoints of the presented procedure. 
The presented protocol is a reproducible and physiologically relevant approach to obtain a bi-ventricular cardiac PV loop recording in a large animal model. This can be applied to a large variety of cardiovascular animal research.

Here we present a closed chest approach to admittance-based bi-ventricular pressure-volume loop recordings in pigs with acute right ventricular dysfunction.

סגור חזה Biventricular לחץ-נפח לולאה הקלטות עם צנתרי כניסה במודל חזירי

Pressure-volume (PV) loop recording enables the state-of-the-art investigation of load-independent variables of ventricular performance. Uni-ventricular ...