Injection Mix and Pronuclear and Cytoplasmic Injections
6:31
Reimplantation
8:21
Results: Microinjection of Oocytes to Generate Genetically Modified Mice
9:49
Conclusion
Trascrizione
The overall goal of this protocol, is to describe the procedures required for the successful generation of genetically modified mice by microinjection of oocytes. This protocol can help researchers and technical staff involved either in the field
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The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.