Results: Immunolabeled and Optically Cleared Kidney Tissue
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Conclusion
Trascrizione
Growing interest in studying large, multi-cellular structures led to the development of this optic clearing technique which allows for a three-dimensional visualization and quantification of large structures within transparent organs. This protoco
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The combination of antibody labeling, optical clearing, and advanced light microscopy allows three-dimensional analysis of complete structures or organs. Described here is a simple method to combine immunolabeling of thick kidney slices, optical clearing with ethyl cinnamate, and confocal imaging that enables visualization and quantification of three-dimensional kidney structures.