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Bioluminescence Resonance Energy Transfer (BRET)-Based Assay for Measuring Interactions of CRAF with 14-3-3 Proteins in Live Cells

805 Views

06:44 min

March 1st, 2024

DOI :

10.3791/66436-v

March 1st, 2024


Transcript

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Bioluminescence Resonance Energy Transfer BRET

Chapters in this video

0:00

Introduction

1:05

Transfection of 293FT Cells with pCMV5‐NanoLuc‐CRAF and pCMV5‐14‐3‐3ζ‐Halo Expression Constructs

3:04

Preparing 293FT Cells Transfected with pCMV5‐NanoLuc‐CRAF and pCMV5‐14‐3‐3ζ‐Halo for BRET Emission Assay

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