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JoVE Journal

Neuroscience

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Spinal Cord Transection in the Larval Zebrafish

11.7K Views

06:57 min

May 21st, 2014

May 21st, 2014

11,692 Views

0:05

Title

1:11

Preparation of Surgery Plates and Micropipettes

2:26

Preparation of Zebrafish Larvae

3:47

Surgery

5:03

Recovery

5:55

Results: Verifying a Complete Transection

6:38

Conclusion

After spinal transection, adult zebrafish have functional recovery by six weeks post-injury. To take advantage of larval transparency and faster recovery, we present a method for transecting the larval spinal cord. After transection, we observe sensory recovery beginning at 2 days post-injury, and C-bend movement by 3 days post-injury.

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