JoVE Journal

Developmental Biology

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Generation of a Human iPSC-Based Blood-Brain Barrier Chip

11.9K Views

10:20 min

March 2nd, 2020

March 2nd, 2020

11,902 Views

0:05

Introduction

1:27

Seeding the "Brain Side" Channel and Differentiating EZ Spheres into Mixed Neural Cultures

3:04

Seeding iBMECs (iPSC-Derived Brain Microvascular Endothelial Cells) into the Bottom Channel to Generate the "Blood Side"

5:27

Initiation of Flow

8:12

Results: Immunocytochemistry and Permeability of the iPSC-Base Blood-Brain Barrier Chip

9:17

Conclusion

文字起こし

This protocol will demonstrate how differentiated induced pluripotent stem cells can be seeded on an organ-on-chip to generate a fully human, personalized, microfluidic blood-brain barrier, which can be used to predict central nervous system drug

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The blood-brain barrier (BBB) is a multicellular neurovascular unit tightly regulating brain homeostasis. By combining human iPSCs and organ-on-chip technologies, we have generated a personalized BBB chip, suitable for disease modeling and CNS drug penetrability predictions. A detailed protocol is described for the generation and operation of the BBB chip.

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