Surface Preparation and Activation of the Coverslip
4:37
Imaging Cell Receptor Forces
5:21
Results: Estimation of Quality and Functionalization of the DNA Probe Surface
6:17
Conclusion
文字起こし
This protocol offers a method to map the molecular forces generated by cells and particularly immune cells. The protocol is critical to labs interested in studying mechanotransduction in mechanobiology. The main advantage of this technique is that
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This paper describes a detailed protocol for using DNA-based tension probes to image the receptor forces applied by immune cells. This approach can map receptor forces >4.7pN in real-time and can integrate forces over time.