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Isolation of Human Primary Valve Cells for In vitro Disease Modeling

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07:31 min

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April 16th, 2021

DOI :

10.3791/62439-v

April 16th, 2021

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2,417 Views

Rolando A. Cuevas¹, Claire C. Chu¹, William J. Moorhead III¹, Ryan Wong¹, Ibrahim Sultan², Cynthia St. Hilaire¹^,³

¹Division of Cardiology, Department of Medicine, and the Pittsburgh Heart, Lung, and Blood Vascular Medicine Institute, University of Pittsburgh, ²Division of Cardiac Surgery, Department of Cardiothoracic Surgery, University of Pittsburgh and Heart and Vascular Institute, University of Pittsburgh Medical Center, ³Department of Bioengineering, University of Pittsburgh

章

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要約

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Human Primary Valve Cells

In Vitro Disease Modeling

Calcific Aortic Valve Disease

Valve Endothelial Cells

Valve Interstitial Cells

Cell Isolation

Tissue Extraction

Collagenase Digestion

Cell Viability

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