Valve Endothelial Cell (VEC) Isolation, Expansion, and Confirmation
4:01
Valve Interstitial Cell (VIC) Isolation, Expansion, and Confirmation
5:40
Results: Analysis of Isolated Human Primary Valve Cells
6:47
Conclusion
Transkript
The isolation of human primary valve endothelial and interstitial cell is critical for understand the underlying mechanism of the pathogenesis of calcific aortic valve disease. When the valve is excised from the native tissue, the cell viability d
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This protocol describes the collection of human aortic valves extracted during surgical aortic valve replacement procedures or from cadaveric tissue, and the subsequent isolation, expansion, and characterization of patient specific primary valve endothelial and interstitial cells. Included are important details regarding the processes needed to ensure cell viability and phenotype specificity.