Measuring Mitochondrial Electron Transfer Complexes in Previously Frozen Cardiac Tissue from the Offspring of Sow: A Model to Assess Exercise-Induced Mitochondrial Bioenergetics Changes

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08:04 min

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August 16th, 2021

DOI :

10.3791/62809-v

August 16th, 2021

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2,458 Views

Daniel Barrera¹, Sierra Upton², Megan Rauch³, Tara Notarianni⁴, Ki Suk Eum⁵, Megan Liberty⁶, Sarmila Venkoba Sah⁷, Robert Liu⁸, Sean Newcomer⁹, Linda E. May¹⁰, Emre Agbas¹¹, Jessica Sage¹², Edina Kosa⁷, Abdulbaki Agbas⁷^,¹³

¹AdventHelath, ²University of Illinois Chicago School of Public Health, ³Lincoln Memorial University, ⁴Children’s Mercy Hospital, ⁵Scripps Clinic, ⁶Cincinnati Children’s Hospital Medical Center, ⁷Kansas City University, ⁸Roblex Rex Veterans Affairs Medical Center, ⁹California State University San Marcos, ¹⁰East Carolina University, ¹¹Massachusetts Institute of Technology, ¹²Boehringer Ingelheim Norway KS, ¹³Heartland Center for Mitochondrial Medicine

章

0:05

Introduction

1:12

Mitochondria Isolation from Frozen Heart Tissue

6:18

Results: Exercise-Induced Changes in Mitochondrial ETC and Supercomplex Formation

7:27

Conclusion

文字起こし

This protocol is significant because it allows users to prepare mitochondria samples from frozen tissues collected from previous studies and archived. This protocol also reduces the use of live animals for tissue harvest. This technique allows use

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要約

Preparation of mitochondria-enriched samples from previously frozen archived solid tissues allowed the investigators to perform both functional and analytical assessments of mitochondria in various experimental modalities. This study demonstrates how to prepare mitochondria-enriched preparations from frozen heart tissue and perform analytical assessments of mitochondria.

さらに動画を探す

Mitochondrial Electron Transfer

Cardiac Tissue

Frozen Tissue

Sow Offspring

Exercise induced Mitochondrial Bioenergetics

Tissue Shredding

Mitochondria Preparation

Tissue Harvesting

Tissue Archiving

Mitochondrial Yield