Results: Analysis of Neurospheres from Adult Zebrafish Brain
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Conclusion
필기록
The overall goal of this procedure is to examine adult neurogenesis using a neurosphere assay derived from the adult zebrafish brain, and to manipulate gene expression in zebrafish neurospheres. So, this method can help to answer a key question in
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Here we provide a reproducible method to examine adult neurogenesis using a neurosphere assay derived from the whole brain or from either the telencephalic, tectal or cerebellar regions of the adult zebrafish brain. Additionally, we describe the procedure to manipulate gene expression in zebrafish neurospheres.