Osmification and Embedding for Electron Microscopy Observation
6:23
Sample Preparation for Ultrathin Sectioning and Observation Using a Transmission Electron Microscope
9:05
Results: Immunolabeling of the Squirrel Monkey Globus Pallidus for ChAT, SERT and TH
11:08
Conclusion
필기록
The overall goal of this experiment is to obtain reliable pre-embedding immunostaining of non-primate brain tissue for electron microscopy. This method can help answer key questions in the neuroanatomy field, such as the synaptic incidence of a gi
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Here, we provide an easy, low-cost, and time-efficient protocol to chemically fix primate brain tissue with acrolein fixative, allowing for long-term preservation that is compatible with pre-embedding immunohistochemistry for transmission electron microscopy.