Force Spectroscopy Experimental Set-up and Acquisition
3:08
Data Analysis
4:48
Measure of Turgor Pressure
6:59
Data Analysis
9:04
Results: AFM Measurements of Cell Wall Mechanical Properties and Turgor Pressure Detection
10:35
Conclusion
필기록
Mechanical properties of plant cells are essential to be taken into account when trying to understand mechanisms underlying development. Atomic force microscopy can be used to measure these properties, and follow the way they change, between organ
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Here, we present atomic force microscopy (AFM), operated as a nano- and micro-indentation tool on cells and tissues. The instrument allows the simultaneous acquisition of 3D surface topography of the sample and its mechanical properties, including cell wall Young's modulus as well as turgor pressure.