Vascular Smooth Muscle Cell Labelling with EdU and Fixing with PFA
1:53
Detection of EdU Using Fluorescence
2:50
Detection of EdU Using Luminescence
4:40
Results: Proliferation of Vascular Smooth Muscle Cells Measurements
6:06
Conclusion
필기록
Proliferation is an important measure of cell function. This protocol allows investigators to develop a sensitive proliferation assay in their labs without the high cost of commercially available kits. The main advantage of this technique is that
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Proliferation is a critical part of cellular function, and a common readout used to assess potential toxicity of new drugs. Measuring proliferation is, therefore, a frequently used assay in cell biology. Here we present a simple, versatile method of measuring proliferation that can be used in adherent and non-adherent cells.