This protocol is capable of producing robust thoracic aortic aneurysms of consistent size and location in mice and serves as a guide for safe access to the murine chest. This protocol is a straightforward, consistent method for producing thoracic
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We describe a surgical protocol to consistently induce robust descending thoracic aortic aneurysms in mice. The procedure involves left thoracotomy, thoracic aorta exposure, and placement of a sponge soaked in porcine pancreatic elastase on the aortic wall.