C. elegans Embryo Semi-High-Throughput Imaging Preparation
3:15
Automated Cropping
4:12
Visualization
5:11
Results: Representative High-Content Imaging of C. elegans Embryogenesis
5:56
Conclusion
필기록
C.elegans embryogenesis takes about 13 hours, and is usually monitored by filming one embryo at a time. Our protocol, however, allows the simultaneous 3D time lapse imaging of 80 to 100 embryos. The ability to collect data for large numbers of dev
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This work describes a semi-high-throughput protocol that allows simultaneous 3D time-lapse imaging of embryogenesis in 80–100 C. elegans embryos in a single overnight run. Additionally, image processing and visualization tools are included to streamline data analysis. The combination of these methods with custom reporter strains enables detailed monitoring of embryogenesis.