Quantification and Quality Check of Tissue Lysates
2:33
Enzymatic Digestion of Proteins
3:57
Desalting of Digested Peptides
5:16
Quantification of Desalted Peptides
5:58
Labeling of Digested Peptides using iTRAQ Reagents
8:14
Liquid Chromatography Set-up
9:25
Mass Spectrometry Set-up
12:30
Representative Results
13:45
Conclusions
필기록
Accurate quantitative measurements of proteins is crucial to comprehend the dynamic and spatial cooperation among proteins to understand the disease back to biology. The overall objective of this experiment is to provide an integrated quantitative
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The described protocol provides an optimized quantitative proteomics analysis of tissue samples using two approaches: label-based and label free quantitation. Label-based approaches have the advantage of more accurate quantitation of proteins, while a label-free approach is more cost-effective and used to analyze hundreds of samples of a cohort.