Targeted proteomic approaches are fast becoming the method of choice for validation of proteins from short-term proteomics-based experiments or from molecular biology-based experiments. Multiple reaction monitoring is one such targeted approach wh
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The protocol aims to introduce the use of a triple quadrupole mass spectrometer for Multiple Reaction Monitoring (MRM) of proteins from clinical samples. We have provided a systematic workflow starting from sample preparation to data analysis for clinical samples with all the necessary precautions to be taken.