Our PLA protocol allows to identify and to quantify endogenous interaction between proteins at membrane contact sites. We have used it to study the interaction between two endoplasmic reticulum proteins at endoplasmic reticulum mitochondrial conta
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The need for new approaches to study membrane contact sites (MCSs) has grown due to increasing interest in studying these cellular structures and their components. Here, we present a protocol that integrates previously available microscopy technologies to identify and quantify intra-organelle and inter-organelle protein complexes that reside at MCSs.