(1) 국립자연과학재단(81973877), (2)한의학의 계승, 혁신 및 발전을 더욱 가속화하기 위한 상하이 3개년 행동계획(ZY(2021-2023)-0201-01), (3) 푸둥 신지역 보건 시스템 푸동 유명 의사 양성 계획(PWRzm2020-15), (4) 한의학 재활 서비스 강화 프로젝트(의료 032)의 재정적 지원에 감사드립니다.

이 연구의 프로토콜은 상하이 중의약 대학 부속 룽화병원 인간 연구 윤리 위원회의 승인을 받았습니다(승인 번호: 2023-LHXS-030). 임상시험 전에 연구에 포함된 각 환자가 서면 동의서를 제공했습니다. 2022년 12월부터 2023년 5월까지 상하이 중의과대학 부속 룽화병원 재활과에서 선정 기준을 충족한 I-II 오십견 환자 총 76명을 무작위로 대조군과 치료군으로 나누어 각 그룹에 38건씩 나누었다. 시술자는 어깨 관절의 해부학적 구조와 특정 수술 방법에 대해 잘 알고 있어야 합니다.
1. 환자 모집
<ol><li>이 연구에 환자를 등록하기 위해 다음 포함 기준을 사용하십시오. 오십견의 진단 기준을 준수합니다. I기 또는 II기 오십견; 성별에 관계없이 40-65세; VAS 점수 ≤ 7; 동시에 다른 치료 없이.</li>
	<li>이 연구에 환자를 포함하지 않으려면 다음 제외 기준을 사용하십시오: 혈액 응고 기능 장애가 있는 환자; 혈당 조절이 불안정한 당뇨병 환자; 임신한 수유부; 치료 부위가 손상되었거나 심각한 감염이 있는 환자; 마취에 알레르기가 있는 환자.</li>
</ol>2. 대조군을 위한 운동 요법
<ol><li>대조군의 환자에게 아래에 설명된 기능적 운동의 치료 과정을19.<ol><li>손 벽 오르기: 환자에게 두 발을 벌리고 벽을 향해 서서 두 손을 들어 벽을 따라 올라가도록 요청합니다. 환자에게 최대 허용 오차로 손을 가장 높은 눈금으로 잡고 각 환자에 대해 기록하도록 요청합니다.</li>
		<li>보조 손: 서 있는 자세에서 환자에게 영향을 받은 상지를 뒤로 신전 상태로 유지하고 건강한 상지로 영향을 받은 상지의 손목을 당겨 반대쪽으로 내부 회전을 수행하도록 요청한 다음 환자가 견딜 수 있는 최대 허용 오차까지 위쪽으로 당기는 상태를 유지합니다.</li>
		<li>진자 흔들기: 서서 구부린 자세에서 환자에게 각 환자의 건강한 팔을 지지하고 영향을 받는 쪽으로 떨어뜨리도록 요청하고 앞뒤, 왼쪽과 오른쪽, 원 동작을 수행합니다. 증상이 호전되면 가동 범위를 넓히고 1-2kg의 물체(물병, 덤벨 등)를 잡고 원을 그리면 스트레칭 운동의 강도를 높일 수 있습니다.</li>
	</ol></li>
	<li>기능적 운동 과정: 대조군의 환자가 4주 동안 매일 30회/4회 벽 등반, 손 보조 및 진자 스윙을 포함한 기능적 운동을 수행하도록 합니다. 알림: 기능적 운동은 허용 범위 내에서 수행해야 합니다. 그렇지 않으면 어깨에 연조직 긴장이 발생하여 상태가 악화될 수 있습니다.</li>
	<li>기능적 운동 중에 가벼운 것에서 무거운 것까지, 작은 것에서 큰 것까지, 단계별로 지속적으로 원칙을 따르십시오. 환자에게 치료 중 모든 일상 활동을 수행할 수 있지만 장기간의 어깨 작업, 무거운 물건 들기 또는 격렬한 활동을 피하도록 요청하십시오.</li>
</ol>3. 치료군의 운동요법
<ol><li>치료군의 환자에게 기능적 운동과 함께 작은 바늘 칼 요법을 실시합니다.</li>
	<li>수술 전 준비<ol><li>재료 준비(그림 1): 일회용 의료용 플라스틱 컵, 의료용 핀셋, 흡수성 면봉, 드레싱 블록, 40mm x 40mm 일회용 멸균 소형 바늘 칼, 마커 펜, II형 피부 소독제, 2% 리도카인 염산염, 주사용 멸균수, 일회용 멸균 주사기, 일회용 멸균 주사 바늘, 고무 수술용 장갑, 접착식 드레싱.</li>
		<li>환자 정보 확인: 환자별 기본 정보(이름, 나이, 환부 어깨)를 확인합니다. 작은 바늘 칼 요법의 과정과 장점을 설명하십시오. 수술 과정에서 환자가 완전히 이해하고 긴장을 풀도록 하십시오.</li>
		<li>환자 준비: 앉은 자세에서 영향을 받은 어깨를 완전히 노출시킵니다.</li>
		<li>포지셔닝(그림 2): 코라코이드 돌기 지점(이두박근의 짧은 머리의 부착 지점), 더 큰 결절(견갑하 근육의 부착 지점), 결핵간 고랑(이두박근의 긴 머리의 부착 지점), 견봉하 윤활낭(극상근의 부착 지점)과 같은 영향을 받는 어깨의 명백한 압통 지점을 표시합니다. 상완골의 더 큰 결절 뒤 2cm(테레스 소근육의 부착 지점)20.</li>
		<li>소독: II형 피부 소독제에 면봉을 준비합니다. 마킹 지점 주위 15cm부터 점차적으로 직경을 줄이면서 피부를 3번 정기적으로 소독합니다.</li>
	</ol></li>
	<li>운영 절차(그림 2)<ol><li>5mL의 2% 리도카인과 5mL의 멸균수를 10mL 주사기에 넣어 10mL 1% 리도카인 작업 용액을 준비합니다.</li>
		<li>22G 바늘을 25G 바늘로 교체합니다(통증을 완화하기 위해 더 얇은 바늘을 사용하십시오). 왼손에는 멸균 장갑을 끼고 오른손에는 주사기를 잡습니다.</li>
		<li>표시에 바늘을 삽입하고 흡인 시 피가 재발하지 않도록 합니다. 표시된 각 지점에 1-2mL의 1% 리도카인 작업 용액을 수직으로 주입합니다.</li>
		<li>작은 바늘 칼 삽입(그림 2): 오른손에 멸균 장갑을 끼고 작은 바늘 칼을 잡습니다. 작은 바늘 칼을 리도카인 주입 부위에 약 1-3cm 깊이로 수직으로 빠르게 삽입합니다. 중요한 혈관과 신경의 손상을 방지하기 위해 작은 바늘 칼날의 날이 근섬유와 평행한지 확인하십시오.</li>
		<li>세로 절개의 3 단계 조작을 수행하고 접착7,15을 벗기고 해제합니다. 환자는 국소 통증과 통증을 느끼고 작업자는 칼 아래에서 저항을 느낄 것입니다.</li>
		<li>작업자가 칼 아래에서 느슨함을 느끼면 바늘을 빼십시오.</li>
	</ol></li>
	<li>수술 후 관리(그림 2)<ol><li>마른 멸균 면봉으로 주사 부위를 1분 동안 누릅니다. 멸균 접착식 드레싱으로 수술 부위를 덮습니다.</li>
		<li>환자가 옷을 잘 입도록 돕습니다. 알림: 의료 폐기물은 의료 폐기물 쓰레기통에 버리십시오.</li>
		<li>수술 후 불편함을 피하기 위해 수술실을 떠나기 전에 환자에게 약 15분 동안 가만히 앉아 있도록 요청하십시오. 출혈과 감염을 방지하기 위해 48시간 후에 제거할 때까지 접착식 드레싱을 건조한 상태로 유지하도록 환자에게 조언합니다.</li>
	</ol></li>
	<li>작은 바늘 칼 요법 과정21: 치료 그룹의 환자에게 일주일에 한 번 총 4개의 세션으로 작은 바늘 칼 요법을 실시합니다. 대조군과 마찬가지로 치료군의 환자에 대해서도 동일한 기능적 운동을 수행합니다.</li>
</ol>4. 개입 후 결과 평가
<ol><li>평가지수 22,23,24 <ol><li>VAS(Visual Analogue Score), CMS(Constant and Murley score), 초음파 검사 시 CHL 두께를 사용하여 결과를 평가합니다. 참고: 기준선 측정은 치료 전과 치료 1주일 후에 수행되었습니다.</li>
	</ol></li>
	<li>평가 도구<ol><li>어깨 관절 동작 측정과 휴대용 컬러 초음파 시스템을 위해 GJJDC01 고정밀 관절 동작 전자 측정기를 사용합니다(그림 3).</li>
	</ol></li>
	<li>평가 도구 작동 방법(그림 3)<ol><li>어깨 운동 범위 측정.<ol><li>ON을 눌러 전원을 켭니다. 피험자에게 어깨를 가능한 최대 각도로 들어 올리도록 요청하십시오. 측정 눈금자를 돌려 측정값을 얻습니다.</li>
			<li>측정기 눈금을 다시 0으로 설정하고 측정을 2번 반복합니다. OFF 를 길게 눌러 전원을 끕니다.</li>
		</ol></li>
		<li>초음파를 이용한 CHL의 두께 측정.<ol><li>전원 버튼을 눌러 초음파 기계를 켭니다. 환자(Patient) 버튼을 누르고 새 환자(New Patient)를 클릭한 다음 피험자의 이름을 입력합니다.</li>
			<li>환자에게 앉은 자세에서 영향을 받은 어깨를 완전히 노출하도록 요청합니다. 환자의 어깨 관절을 수동적으로 움직여 CHL을 찾습니다. 초음파 프로브를 CHL에 맞춥니다.</li>
			<li>Freeze 버튼을 눌러 초음파 이미지를 저장합니다. 캘리퍼 버튼을 눌러 CHL 두께를 테스트합니다. 측정값을 기록하고 끕니다.</li>
		</ol></li>
	</ol></li>
</ol>5. 통계 분석
<ol><li>계량형 변수를 평균 ± 표준 편차(SD)로 표시합니다. 독립 표본 t-검정을 사용하여 두 그룹 간의 차이를 비교합니다. 모든 통계적 검정은 양측적이었고 P<0.05는 통계적으로 유의한 것으로 간주되었습니다. 모든 분석은 통계 소프트웨어를 사용하여 수행되었습니다.</li>
</ol>

오십견 치료에서 작은 바늘 칼 요법의 치료 효능

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Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Bibliometrics+and+knowledge+graph+analysis+of+small+needle+knife.">Bibliometrics and knowledge graph analysis of small needle knife.</a> Chinese J Lib Info Traditional Chinese Med. 45 (6), 13-17 (2021).</li><li>Qin, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Outcomes+of+visualized+puncture+needle+and+small+needle+knife+therapy+in+primary+frozen+shoulder+based+on+multimodal+ultrasound+imaging.">Outcomes of visualized puncture needle and small needle knife therapy in primary frozen shoulder based on multimodal ultrasound imaging.</a> J Healthc Eng. 2022, 1076112 (2022).</li><li>Zeng, X., Ding, Z. H., Fu, L. Q., Liu, S. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Research+progress+of+ultrasound+small+needle+knife+visualization.">Research progress of ultrasound small needle knife visualization.</a> J Acupuncture Res. 46 (6), 546-548 (2019).</li><li>Mertens, M. G., Meert, L., Struyf, F., Schwank, A., Meeus, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exercise+therapy+is+effective+for+improvement+in+range+of+motion,+function,+and+pain+in+patients+with+frozen+shoulder:+A+systematic+review+and+meta-analysis.">Exercise therapy is effective for improvement in range of motion, function, and pain in patients with frozen shoulder: A systematic review and meta-analysis.</a> Arch Phys Med Rehabil. 103 (5), 998-1012 (2022).</li><li>Xu, W. S., Liu, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+observation+of+&amp;#34;C&amp;#34;+shape+release+by+small+needle+knife+in+treatment+of+frozen+shoulder.">Clinical observation of &amp;#34;C&amp;#34; shape release by small needle knife in treatment of frozen shoulder.</a> J Liaoning Uni Traditional Chinese Med. 21 (9), 185-188 (2019).</li><li>Liang, C. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Basic+research+on+a+recommended+program+of+clinical+practice+guidelines+for+small+needle+knife+treatment+of+frozen+shoulder.">Basic research on a recommended program of clinical practice guidelines for small needle knife treatment of frozen shoulder.</a> Beijing University of Chinese Medicine. , (2012).</li><li>Tian, Z. R., Yao, M., Wang, Y. J., Cui, X. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+and+application+of+Constant-Murley+shoulder+joint+Rating+scale+in+Chinese.">Development and application of Constant-Murley shoulder joint Rating scale in Chinese.</a> Chinese Med Bone Sett. 31 (5), 20-25 (2019).</li><li>Yin, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Application+of+visual+diagnosis+and+treatment+technique+(ultrasound)+in+diagnosis+and+treatment+of+frozen+shoulder+and+evaluation+of+curative+effect.">Application of visual diagnosis and treatment technique (ultrasound) in diagnosis and treatment of frozen shoulder and evaluation of curative effect.</a> Chinese J Med Ultrasound. 16 (11), 810-814 (2019).</li><li>Fan, Z. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+measurement+of+coracohumeral+ligament+thickness+in+diagnosis+and+evaluation+of+the+curative+effect+of+frozen+shoulder.">The role of measurement of coracohumeral ligament thickness in diagnosis and evaluation of the curative effect of frozen shoulder.</a> Soochow University. , (2018).</li><li>Reeves, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+natural+history+of+the+frozen+shoulder+syndrome.">The natural history of the frozen shoulder syndrome.</a> Scand J Rheumatol. 4 (4), 193-196 (1975).</li><li>Xu, Y., Bonar, F., Murrell, G. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Enhanced+expression+of+neuronal+proteins+in+idiopathic+frozen+shoulder.">Enhanced expression of neuronal proteins in idiopathic frozen shoulder.</a> J Shoulder Elbow Surg. 21 (10), 1391-1397 (2012).</li><li>Rodeo, S. A., Hannafin, J. A., Tom, J., Warren, R. F., Wickiewicz, T. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immunolocalization+of+cytokines+and+their+receptors+in+adhesive+capsulitis+of+the+shoulder.">Immunolocalization of cytokines and their receptors in adhesive capsulitis of the shoulder.</a> J Orthop Res. 15 (3), 427-436 (1997).</li><li>Green, H. D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+genome-wide+association+study+identifies+5+loci+associated+with+frozen+shoulder+and+implicates+diabetes+as+a+causal+risk+factor.">A genome-wide association study identifies 5 loci associated with frozen shoulder and implicates diabetes as a causal risk factor.</a> PLoS Genet. 17 (6), e1009577 (2021).</li><li>Hand, G. C., Athanasou, N. A., Matthews, T., Carr, A. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+pathology+of+frozen+shoulder.">The pathology of frozen shoulder.</a> J Bone Joint Surg Br. 89 (7), 928-932 (2007).</li><li>Lho, Y. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inflammatory+cytokines+are+overexpressed+in+the+subacromial+bursa+of+frozen+shoulder.">Inflammatory cytokines are overexpressed in the subacromial bursa of frozen shoulder.</a> J Shoulder Elbow Surg. 22 (5), 666-672 (2013).</li><li>Han, Y. L., Sun, W. Q., Huo, S. F., Xu, Z. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Recent+therapeutic+effect+and+mechanism+of+small+needle+knife+in+the+treatment+of+patients+with+cold-dampness-blocking+frozen+shoulder.">Recent therapeutic effect and mechanism of small needle knife in the treatment of patients with cold-dampness-blocking frozen shoulder.</a> World of Chinese Medicine. 13 (5), 1254-1257 (2018).</li><li>Zhou, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+small+needle+knife+therapy+on+expression+of+IL-1&#946;,+TNF-&#945;,+and+ASIC1+in+local+tissue+of+rabbits+with+frozen+shoulder.">Effect of small needle knife therapy on expression of IL-1&#946;, TNF-&#945;, and ASIC1 in local tissue of rabbits with frozen shoulder.</a> Shanghai J Acupuncture Moxibustion. 40 (6), 762-768 (2021).</li><li>Zhang, Z. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+small+needle+knife+and+local+closure+on+pain+mediators+and+inflammatory+factors+in+patients+with+frozen+shoulder.">Effect of small needle knife and local closure on pain mediators and inflammatory factors in patients with frozen shoulder.</a> Clin Res Chinese Med. 10 (8), 82-84 (2018).</li><li>Bunker, T. D., Reilly, J., Baird, K. S., Hamblen, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expression+of+growth+factors,+cytokines+and+matrix+metalloproteinases+in+frozen+shoulder.">Expression of growth factors, cytokines and matrix metalloproteinases in frozen shoulder.</a> J Bone Joint Surg Br. 82 (5), 768-773 (2000).</li><li>Millar, N. L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Frozen+shoulder.">Frozen shoulder.</a> Nat Rev Dis Primers. 8 (1), 59 (2022).</li><li>Li, H. Y., Chen, S. Y., Zhai, W. T., Chen, J. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expression+of+TGF-&#946;+and+MMPs+in+capsula+tissue+of+frozen+shoulder+patients.">Expression of TGF-&#946; and MMPs in capsula tissue of frozen shoulder patients.</a> J Shanghai Jiaotong Uni. 29 (11), 1363-1366 (2009).</li><li>Cher, J. Z. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Alarmins+in+Frozen+Shoulder:+A+Molecular+Association+Between+Inflammation+and+Pain.">Alarmins in Frozen Shoulder: A Molecular Association Between Inflammation and Pain.</a> Am J Sports Med. 46 (3), 671-678 (2018).</li><li>Wang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+study+on+the+treatment+of+frozen+shoulder+in+rats+with+&amp;#34;C&amp;#34;+shaped+small+needle+knife.">Experimental study on the treatment of frozen shoulder in rats with &amp;#34;C&amp;#34; shaped small needle knife.</a> Hubei Uni Chinese Med. , (2020).</li><li>Bai, Y., Chen, B., Qin, Y. N., Du, Y. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thoughts+on+constructing+the+core+outcome+index+set+of+clinical+research+on+frozen+shoulder.">Thoughts on constructing the core outcome index set of clinical research on frozen shoulder.</a> Chinese Med J. 36 (1), 69-73 (2019).</li><li>Buchbinder, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Preliminary+Core+Domain+Set+for+Clinical+Trials+of+Shoulder+Disorders:+A+Report+from+the+OMERACT+2016+Shoulder+Core+Outcome+Set+Special+Interest+Group.">A Preliminary Core Domain Set for Clinical Trials of Shoulder Disorders: A Report from the OMERACT 2016 Shoulder Core Outcome Set Special Interest Group.</a> J Rheumatol. 44 (12), 1880-1883 (2017).</li><li>Fan, Z. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+measurement+of+coracohumeral+ligament+thickness+in+diagnosis+and+evaluation+of+curative+effect+of+frozen+shoulder.">The role of measurement of coracohumeral ligament thickness in diagnosis and evaluation of curative effect of frozen shoulder.</a> Soochow University. , (2018).</li><li>Xiu, Z. B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinical+guidelines+of+small+needle+knife+for+knee+osteoarthritis.">Clinical guidelines of small needle knife for knee osteoarthritis.</a> J Rehabil. 33 (3), 193-201 (2019).</li></ol>

저자는 이해 상충이 없다고 보고합니다.

이 연구에서 우리는 작은 바늘 칼 요법이 통증 증상을 현저히 완화하고, 어깨 관절 기능을 개선하고, CHL의 두께를 줄이고, I-II 오십견 환자의 삶의 질을 향상시킨다는 것을 발견했습니다. 작은 바늘 칼 요법은 오십견에 정확한 효과가 있었습니다. 그러나 수술 현장은 논란의 여지가 있습니다. 다양한 지식 배경으로 인해 작은 바늘 칼로 오십견을 치료하기 위해 많은 수술 부위를 선택할 수 있습니?...

오십견은 통증과 능동적 및 수동적 어깨 운동의 제한을 특징으로 하는 질환으로 환자의 업무와 생활에 큰 불편을 초래한다1. 오십견의 전 세계 발병률은 2%-5%2,3이며 남성보다 여성이 더 높으며 특정 유전적 경향을 보인다4. 오십견 환자의 대부분은 40-60세이다5. 더욱이 오십견은 회복 주기가 길고 임상에서 치료에 대한 합의가 이루어지지 않아 사회적 부담이 크다.
오십견의 주요 치료법으로는 약초의 경구 또는 외용, 침술, 마사지, 기능적 운동 등이 있다6. 그러나 치료 빈도가 다양하고 시간이 오래 소요되어 환자의 순응도가 낮으며, 통증 및 기능적 제한을 개선하기 위한 환자의 긴급한 요구를 충족시키지 못하고 있습니다. 따라서 오십견 환자의 임상적 관리에서 보다 효과적인 치료법을 찾는 것이 시급하다.
작은 바늘 칼 요법은 최근 수십 년 동안 중국 전통 의학 분야에서 급속히 부상한 중국 전통 의학의 특별하고 효과적인 외부 치료법입니다7. 빠른 효과, 확실한 치료 효과 및 높은 반복성의 장점이 있습니다. 또한 조작 기기는 저렴하고 구하기 쉽고 조작 과정이 간단하며 방법은 환경 및 조건에 의해 제한되지 않습니다. 작은 바늘 칼 치료 후 환자의 정상적인 일과 생활은 미세 절개가 신체에 손상을 입히거나 감염을 일으키거나 기타 부작용을 일으키지 않고 빠르게 회복되기 때문에 영향을 받지 않습니다 8,9,10,11. 체계적 문헌고찰에 따르면 오십견에 대한 작은 바늘 칼의 효능은 침술, 마사지 및 한의학 경구에 비해 유의하게 우수했다12,13. 현대 연구에 따르면 오십견의 작은 바늘 칼 치료의 메커니즘은 경락과 측부를 준설하고, 기와 혈액의 순환을 촉진하고, 병변 조직을 자르고 벗기고, 유착을 제거하고, 조직 복구를 촉진하고, 인체의 동적 균형을 회복하는 것입니다14,15.
여기에 제시된 프로토콜은 환자 관리, 재료 준비, 위치 지정, 수술 및 수술 후 관리를 포함하여 오십견 치료에서 작은 바늘 칼의 작동을 보여줍니다. 이 프로토콜의 목적은 오십견 환자의 통증과 기능적 제한을 완화하고 생활 능력을 향상시키는 것입니다.
소바늘칼 치료법의 발달로 목, 어깨, 허리, 다리 통증 및 기타 근육 및 뼈 질환의 치료에서 내과, 외과, 부인과, 소아과 및 기타 질병으로 그 적용이 확대되어 재활 의학 분야에서 점점 더 중요한 역할을 하고 있습니다16. 최근 몇 년 동안 작은 바늘 칼은 초음파 유도 작은 바늘 칼 시각화 요법의 적용으로 인해 보다 정확하고 과학적이며 안전한 치료 효능을 보여주며, 그 개발은 새로운 단계에 진입했습니다17,18.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Disposable sterile injection needle</td>
			<td>Shanghai Mishava Medical Industry Co., Ltd</td>
			<td>601900973</td>
			<td>Type specification:0.5 x 38 RWLB</td>
		</tr>
		<tr>
			<td>Disposable sterile small needle-knife</td>
			<td>Wujiang Yunlong Medical equipment Co., Ltd</td>
			<td>PR-4040</td>
			<td>Type specification:40 mm x 40 mm&#160;</td>
		</tr>
		<tr>
			<td>Disposable sterile syringe</td>
			<td>Shandong Weigo Group Medical polymer Products Co., Ltd</td>
			<td>601909174</td>
			<td>Type specification:10 mL</td>
		</tr>
		<tr>
			<td>Lidocaine hydrochloride</td>
			<td>Shanghai Hefeng Pharmaceutical Co., Ltd</td>
			<td>6904996104121</td>
			<td>Type specification:5 mL</td>
		</tr>
		<tr>
			<td>Marker pen</td>
			<td>Zebra Trading (Shenzhen) Co., Ltd</td>
			<td>4901681518111</td>
			<td>Type specification:1.0 mm/0.5 mm</td>
		</tr>
		<tr>
			<td>Sterile absorbent cotton ball</td>
			<td>Shanghai Honglong Medical Supplies Equipment Co., Ltd</td>
			<td>601905637</td>
			<td>Type specification:0.3&#160; 25 g</td>
		</tr>
		<tr>
			<td>Sterile disposable medical plastic cup</td>
			<td>Shanghai Honglong Medical Supplies Equipment Co., Ltd</td>
			<td>709008633</td>
			<td>Type specification:Waist-shaped disc</td>
		</tr>
		<tr>
			<td>Sterile dressing block</td>
			<td>Ningbo Haishu Shenyuan Medical Materials Co.,&#160; Ltd</td>
			<td>601909470</td>
			<td>Type specification:7.5 cm x 7.5 cm x 8 floors</td>
		</tr>
		<tr>
			<td>Sterile medical tweezers</td>
			<td>Shanghai Honglong Medical Supplies Equipment Co.,Ltd</td>
			<td>603917444</td>
			<td>Type specification:Small size</td>
		</tr>
		<tr>
			<td>Sterile rubber surgical gloves</td>
			<td>Guilin Hengbao health protection Co.,&#160; Ltd</td>
			<td>6971787071320</td>
			<td>Type specification:6.5</td>
		</tr>
		<tr>
			<td>Sterile self-adhesive dressing</td>
			<td>Shanghai ISO Medical Products Co., Ltd</td>
			<td>601909414</td>
			<td>Type specification:10 cm x 15 cm</td>
		</tr>
		<tr>
			<td>Sterile water for injection</td>
			<td>Shanghai Xinyi Jinzhu Pharmaceutical Co., Ltd</td>
			<td>6938493300953</td>
			<td>Type specification:5 mL</td>
		</tr>
		<tr>
			<td>Type II skin disinfectant</td>
			<td>Shanghai Likang Disinfection high-tech Co., Ltd</td>
			<td>6909157000359</td>
			<td>Type specification:500 mL/ bottle</td>
		</tr>
		<tr>
			<td>Ultrasound</td>
			<td>Suzhou Daer Medical Equipment Co., Ltd</td>
			<td>100018857</td>
			<td>uSmart3300 portable color ultrasound system</td>
		</tr>
	</tbody>
</table>

clinical efficacy of small needle knife therapy on stage i-ii frozen shoulder

오십견은 어깨 관절의 긴장, 외상 및 기타 원인으로 인해 발생하는 어깨의 무균성 염증성 질환의 일종으로 어깨 관절 통증과 기능 제한을 초래합니다. 여기에 제시된 프로토콜은 환자 관리, 재료 준비, 위치 지정, 수술 및 수술 후 관리를 포함하여 오십견 치료에서 작은 바늘 칼의 작동을 보여줍니다. 이 프로토콜의 목적은 오십견 환자의 통증과 기능적 제한을 완화하고 생활 능력을 향상시키는 것입니다. 본 연구에서는 선정 기준을 충족하는 I-II기 오십견 환자 76명을 무작위로 대조군과 치료군(n=38)으로 나누었다. 대조군의 환자는 기능적 운동을 받았고, 치료군은 기능적 운동과 함께 작은 바늘 칼 요법을 받았다. 초음파에서 시각적 아날로그 점수(VAS), 상수 및 머리 점수(CMS), 코라코상완 인대(CHL)의 두께를 평가했습니다. 작은 바늘 칼 치료 후 VAS 점수는 대조군(5.49 ± 0.65)보다 치료군(5.11 ± 0.89)에서 유의하게 낮았습니다. t = -2.065, p<0.05); CMS 점수는 대조군(60.97 ± 6.00)보다 치료군(64.72 ± 4.78)에서 유의하게 높았다. t=2.947, p<0.05); CHL 두께는 대조군(2.57 ±± 0.42; t=-2.117, p<0.05)입니다. 이러한 결과는 작은 바늘 칼이 통증 증상을 현저히 완화하고, 어깨 기능을 개선하고, CHL 두께를 줄이고, 삶의 질을 향상시켜 I-II 기 오십견 환자에게 상당한 치료 효능을 나타낸다는 것을 나타냅니다.

대조군에서는 1명의 환자가 통증 악화로 인해 다른 치료(관절 내 스테로이드 주사)를 받았기 때문에 임상시험을 종료하고, 대조군의 나머지 37명은 최종 통계 분석에 포함시켰다. 치료군 2명은 초음파에서 CHL이 검출되지 않아 검사를 생략하고 나머지 36명의 환자를 최종 통계분석에 들어갔다.
기준선 특성 두 그룹 간의 성별, 연령, 환부, 질병 경과 및 병기의 차?...

Watch this Scientific Journal Video about Treating Frozen Shoulder with Small Needle Knife Therapy at JoVE.com

저자 스포트라이트: 작은 바늘 칼 요법으로 오십견 치료 

여기에 제시된 프로토콜은 자세한 수술 방법을 설명하고 오십견 치료에 작은 바늘 칼의 효과를 증명합니다.

I-II 오십견에 대한 작은 바늘 칼 요법의 임상적 효능

Frozen shoulder is a kind of aseptic inflammatory disease of the shoulder caused by strain, trauma, and other reasons, resulting in shoulder joint pain ...

본 연구는 오십견 환자에 대한 작은 바늘 칼의 치료 효능과 통증 기능 및 생활 능력의 개선에 대한 증거를 조사했습니다. 우리의 프로토콜은 오십견 환자에서 작은 바늘 칼의 자세한 수술 과정을 다룹니다. 우리의 프로토콜은 더 빠르고 확실한 치료 효과, 높은 반복성, 더 넓은 가용성의 장점이 있으며 환자의 신체적 상해가 적습니다.

clinical-efficacy-small-needle-knife-therapy-on-stage-i-ii-frozen

Research

JoVE Journal

Medicine

Clinical Efficacy of Small Needle Knife Therapy on Stage I-II Frozen Shoulder

1.2K 조회수.  Shanghai University of Traditional Chinese Medicine. 본 연구는 오십견 환자에 대한 작은 바늘 칼의 치료 효능과 통증 기능 및 생활 능력의 개선에 대한 증거를 조사했습니다. 우리의 프로토콜은 오십견 환자에서 작은 바늘 칼의 자세한 수술 과정을 다룹니다. 우리의 프로토콜은 더 빠르고 확실한 치료 효과, 높은 반복성, 더 넓은 가용성의 장점이 있으며 환자의 신체적 상해가 적습니다.

비디오: 저자 스포트라이트: 작은 바늘 칼 요법으로 오십견 치료 

Reverse Total Shoulder Arthroplasty

Reverse total shoulder arthroplasty was initially approved for use in rotator cuff arthropathy and well as chronic pseudoparalysis without arthritis in patients who were not appropriate for tendon transfer reconstructions. Traditional surgical options for these patients were limited and functional results were sub-optimal and at times catastrophic. The use of reverse shoulder arthroplasty has been found to effectively restore these patients function and relieve symptoms associated with their disease. The procedure can be done through two approaches, the deltopectoral or the superolateral. Complication rates associated with the use of the prosthesis have ranged from 8-60% with more recent reports trending lower as experienced is gained. Salvage options for a failed reverse shoulder prosthesis are limited and often have significant associated disability. Indications for the use of this prosthesis continue to be evaluated including its use for revision arthroplasty, proximal humeral fracture and tumor. Careful patient selection is essential because of the significant risks associated with the procedure.

Reverse total shoulder arthroplasty is indicated for the treatment of conditions that cannot be treated with conventional arthroplasty or other procedures. These primarily include degenerative and incapacitating conditions with irreparable rotator cuff and loss of the normal biomechanical coupling of the shoulder.

총 숄더 Arthroplasty를 역방향

Reverse total shoulder arthroplasty was initially approved for use in rotator cuff arthropathy and well as chronic pseudoparalysis without arthritis in ...

연구

의학

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

In recent years, there has been the difficulty in finding more effective therapies against cancer with less systemic side effects. Therefore Photodynamic Therapy is a novel approach for a more tumor selective treatment.
Photodynamic Therapy (PDT) that makes use of a nontoxic photosensitizer (PS), which, upon activation with light of a specific wavelength in the presence of oxygen, generates oxygen radicals that elicit a cytotoxic response1. Despite its approval almost twenty years ago by the FDA, PDT is nowadays only used to treat a limited number of cancer types (skin, bladder) and nononcological diseases (psoriasis, actinic keratosis)2.
The major advantage of the use of PDT is the ability to perform a local treatment, which prevents systemic side effects. Moreover, it allows the treatment of tumors at delicate sites (e.g. around nerves or blood vessels). Here, an intraoperative application of PDT is considered in osteosarcoma (OS), a tumor of the bone, to target primary tumor satellites left behind in tumor surrounding tissue after surgical tumor resection. The treatment aims at decreasing the number of recurrences and at reducing the risk for (postoperative) metastasis.
In the present study, we present in vitro PDT procedures to establish the optimal PDT settings for effective treatment of widely used OS cell lines that are used to reproduce the human disease in well established intratibial OS mouse models. The uptake of the PS mTHPC was examined with a spectrophotometer and phototoxicity was provoked with laser light excitation of mTHPC at 652 nm to induce cell death assessed with a WST-1 assay and by the counting of surviving cells. The established techniques enable us to define the optimal PDT settings for future studies in animal models. They are an easy and quick tool for the evaluation of the efficacy of PDT in vitro before an application in vivo.

Cytotoxic Efficacy of Photodynamic Therapy in Osteosarcoma Cells In Vitro

The protocol reported here allows the evaluation of the efficacy of photodynamic therapy (PDT) in cell lines and the optimization of the PDT settings before applying the therapy in animal models.

골육종 세포의 광 역학 치료의 세포 독성 효능<em&gt; 체외</em

In recent years, there has been the difficulty in finding more effective therapies against cancer with less systemic side effects. Therefore Photodynamic ...

Preparation and Respirometric Assessment of Mitochondria Isolated from Skeletal Muscle Tissue Obtained by Percutaneous Needle Biopsy

Respirometric profiling of isolated mitochondria is commonly used to investigate electron transport chain function. We describe a method for obtaining samples of human Vastus lateralis, isolating mitochondria from minimal amounts of skeletal muscle tissue, and plate based respirometric profiling using an extracellular flux (XF) analyzer. Comparison of respirometric profiles obtained using 1.0, 2.5 and 5.0 &#956;g of mitochondria indicate that 1.0 &#956;g is sufficient to measure respiration and that 5.0 &#956;g provides most consistent results based on comparison of standard errors. Western blot analysis of isolated mitochondria for mitochondrial marker COX IV and non-mitochondrial tissue marker GAPDH indicate that there is limited non-mitochondrial contamination using this protocol. The ability to study mitochondrial respirometry in as little as 20 mg of muscle tissue allows users to utilize individual biopsies for multiple study endpoints in clinical research projects.

Methods for biopsy of Vastus lateralis, preparation of purified mitochondria, and respirometric profiling are described. The use of small muscle volume makes this technique suitable for clinical research applications.

경피적 바늘 생검에 의해 획득 골격근 조직에서 고립 된 미토콘드리아의 준비 및 호흡계 평가

Respirometric profiling of isolated mitochondria is commonly used to investigate electron transport chain function. We describe a method for obtaining ...

A Clinical Trial Assessing the Safety, Efficacy, and Delivery of Olive-Oil-Based Three-Chamber Bags for Parenteral Nutrition

Limited evidence exists to precisely estimate efficacy and safety differences between parenteral nutrition (PN) prepared using olive-oil-based three-chamber bags (3CBs) and soybean-oil-based compounded bags (CoBs) in hospitalized adult patients. We designed a multicenter, randomized, prospective, open-label, noninferiority protocol to compare the efficacy, safety, and distribution of olive-oil-based 3CBs and soybean-oil-based CoB formulations in adult Chinese patients requiring PN during surgical intervention. Subjects were randomized to receive either one of the study treatments using an interactive voice or web-based recognition system in accordance with the randomization code. Randomization was further stratified based on the study site and surgical category. Both treatment groups received similar amounts of calories and protein. In addition, the two study treatments contained a similar composition of the amino-acid component. The only difference between the two PN formulations was the lipid constitution. The duration of administration of study treatments was a minimum of 5 days up to a maximum of 14 days after the surgical procedure. The primary efficacy endpoint was serum prealbumin levels on day 5 of the study. Noninferiority was proved if the anti-log of the lower bound of the 95% confidence interval (CI) of the treatment difference was at least 0.80. Other efficacy measures included treatment preparation time; duration to achieve tolerability of oral nutrition; associated infectious complications; length of hospitalization; and laboratory assessment of markers of nutrition, inflammation, metabolism, and oxidative stress. A total of 458 patients were enrolled in the study. The results showed that olive-oil-based 3CBs were non-inferior to soybean-based CoBs, besides being well tolerated. The infection rate was found to be significantly lower in the olive-oil-based 3CB group. Thus, this study may be used as a reference for future research on lipid emulsion and 3CBs.

Here, we present a protocol for a study comparing the efficacy, safety, and delivery of olive-oil-based 3CB and soybean-oil-based CoB formulations in adults requiring parenteral nutrition. The results revealed that olive-oil-based 3CBs is non-inferior and well tolerated compared to soybean formulations.

Limited evidence exists to precisely estimate efficacy and safety differences between parenteral nutrition (PN) prepared using olive-oil-based ...

Proton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies

Radiation therapy is a frequently used modality for the treatment of solid cancers. Although the mechanisms of cell kill are similar for all forms of radiation, the in vivo properties of photon and proton beams differ greatly and maybe exploited to optimize clinical outcomes. In particular, proton particles lose energy in a predictable manner as they pass through the body. This property is used clinically to control the depth at which the proton beam is terminated, and to limit radiation dose beyond the target region. This strategy can allow for substantial reductions in radiation dose to normal tissues located just beyond a tumor target. However, the degradation of proton energy in the body remains highly sensitive to tissue density. As a consequence, any changes in tissue density during the course of treatment may significantly alter proton dosimetry. Such changes may occur through alterations in body weight, respiration, or bowel filling/gas, and may result in unfavorable dose deposition. In this manuscript, we provide a detailed method for the delivery of proton therapy using both passive scatter and pencil beam scanning techniques for prostate cancer. Although the described procedure directly pertains to prostate cancer patients, the method may be adapted and applied for the treatment of virtually all solid tumors. Our aim is to equip readers with a better understanding of proton therapy delivery and outcomes in order to facilitate the appropriate integration of this modality during cancer therapy.

The fundamentals of radiation planning and delivery for proton therapy using prostate cancer as a model are presented. The application of these principles to other selected disease sites highlights how proton radiotherapy may enhance clinical outcomes for cancer patients.

양성자 치료 배달 및 선택 고체 종양 악성 종양에서의 임상 응용

Radiation therapy is a frequently used modality for the treatment of solid cancers. Although the mechanisms of cell kill are similar for all forms of ...

A Pre-clinical Rat Model for the Study of Ischemia-reperfusion Injury in Reconstructive Microsurgery

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many areas of biomedical research due to its cost-effectiveness and its translation to humans. This protocol describes a method to create a preclinical free skin flap model in rats with ischemia-reperfusion injury. The described 3 cm x 6 cm rat free skin flap model is easily obtained after the placement of several vascular ligatures and the section of the vascular pedicle. Then, 8 h after the ischemic insult and completion of the microsurgical anastomosis, the free skin flap develops the tissue damage. These ischemia-reperfusion injury-related damages can be studied in this model, making it a suitable model for evaluating therapeutic agents to address this pathophysiological process. Furthermore, two main monitoring techniques are described in the protocol for the assessment of this animal model: transit-time ultrasound technology and laser speckle contrast analysis.

Here, we describe a pre-clinical animal model for studying the pathophysiology of ischemia-reperfusion injury in reconstructive microsurgery. This free skin flap model based on the superficial caudal epigastric vessels in the rat may also allow for the evaluation of different therapies and compounds to counteract ischemia-reperfusion injury-related damage.

재건 미세 수술에서 허혈 재관류 손상의 연구를위한 전 임상 쥐 모델

Ischemia-reperfusion injury is the main cause of flap failure in reconstructive microsurgery. The rat is the preferred preclinical animal model in many ...

Large Animal Model for Evaluating the Efficacy of the Gene Therapy in Ischemic Heart

Coronary artery disease is one of the significant causes of mortality and morbidity worldwide. Despite the progression of current therapeutics, a considerable proportion of coronary artery disease patients remain symptomatic. Gene therapy-mediated therapeutic angiogenesis offers a novel therapeutic method for improving myocardial perfusion and relieving symptoms. Gene therapy with different angiogenic factors has been studied in few clinical trials. Due to the novelty of the method, the progress of myocardial gene therapy is a continuous path from bench to bedside. Therefore, large animal models are needed for evaluating the safety and efficacy. The more the large animal model identifies the original disease and the endpoints used in clinics, the more predictable outcomes are from clinical trials. Here, we introduce a large animal model for evaluating the efficacy of the gene therapy in the ischemic porcine heart. We use clinically relevant imaging methods such as ultrasound imaging and 15H2O-PET. For targeting the gene transfers into the desired area, electroanatomical mapping is used. The aim of this method is: (1) to mimic chronic coronary artery disease, (2) to induce therapeutic angiogenesis at hypoxic areas of the heart, and (3) to evaluate the safety and efficacy of the gene therapy by using relevant endpoints.

Myocardial gene therapy for ischemic heart disease holds great promise for future therapeutics. Here, we introduce a large animal model for evaluating the efficacy of gene therapy in the ischemic heart.

허혈성 심장에서 유전자 치료의 효능을 평가하기 위한 거대 동물 모델

Coronary artery disease is one of the significant causes of mortality and morbidity worldwide. Despite the progression of current therapeutics, a ...

Subconjunctival Administration of Adeno-associated Virus Vectors in Small Animal Models

Ocular diseases include a wide range of inherited genetic and acquired disorders that are appealing targets for local drug delivery due to their relative ease of accessibility via multiple administration routes. Subconjunctival (SCJ) injections offer advantages over other intraocular administration routes as they are simple, safe, and usually performed in an outpatient setting. SCJ injections in small animals usually require the assistance of an operating microscope due to the size of the eye. Previous work has demonstrated that SCJ injection of specific adeno-associated virus (AAV) serotypes is a valid gene delivery strategy for targeted transduction of the ocular surface, eye muscle, cornea, and optic nerve, providing a potential approach for the treatment of many ocular diseases.
Herein, a detailed protocol is presented for SCJ injections in a mouse model using an injection system consisting of a programmable infusion/withdrawal syringe pump (which allows for consistent and precise injection speed and pressure) and a gastight removable syringe coupled with microinjection needles. The injection system is also adaptable for other intraocular administration routes such as intrastromal, intracameral, intravitreal, and subretinal injections in small animals. Although the delivery of adeno-associated viral vectors for ocular gene therapy studies is described, the protocol herein can also be adapted for a variety of ophthalmic solutions in small animal models. The key practical steps in the administration route, setup for the injection platform, preparation of the injection, and tips from direct experience will be discussed in detail. In addition, common validation techniques for AAV delivery confirmation to the desired tissues will also be briefly discussed.

In this manuscript, subconjunctival injection is demonstrated as a valid vector delivery method for ocular tissues in mice using an injection system consisting of an infusion/withdrawal syringe pump and a gastight removable syringe coupled with microinjection needles. This injection system is also adaptable for other intraocular administration routes.

작은 동물 모델에서 아데노 관련 바이러스 벡터의 결막하 투여

Ocular diseases include a wide range of inherited genetic and acquired disorders that are appealing targets for local drug delivery due to their relative ...

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration

The goal of this article is to present the application of a 24 G cannula and 3-0 polypropylene suture as a simple method for the exploration of the vas deferens. During the exploration of the vas deferens, a 24 G cannula needle was used to puncture it. The fluid in the smear confirmed the presence of sperm, to determine whether or not there was concomitant obstruction at the junction of the epididymis and the vas deferens. Then, a 3-0 polypropylene suture (this suture specification has the advantages of a smooth surface, robust quality, and can be passed through a 24 G cannula needle) was passed through the cannula needle to probe the location of the obstructed site. With this technique, exploration of the vas deferens could be more targeted and accurate.

This protocol presents the clinical application of a 24 G cannula and 3-0 polypropylene suture as a simple and effective method for the exploration of the vas deferens.

정관 탐사에서 24g 캐뉼라 바늘 및 3-0 폴리프로필렌 봉합사의 임상 적용

The goal of this article is to present the application of a 24 G cannula and 3-0 polypropylene suture as a simple method for the exploration of the vas ...

쥐 모델에서 FSN 요법 개선: 불편함 최소화 및 효율성 극대화

Efficacy of Fu's Subcutaneous Needling on Sciatic Nerve Pain: Behavioral and Electrophysiological Changes in a Chronic Constriction Injury Rat Model

Fu&#39;s subcutaneous needling (FSN), an invented acupuncture technique from traditional Chinese medicine, is used worldwide for pain relief. However, the mechanisms of action are still not fully understood. During FSN treatment, the FSN needle is inserted and retained in the subcutaneous tissues for a long duration with a swaying movement. However, challenges arise from maintaining a posture while manipulating FSN in animal models (e.g., rats) for researchers. Uncomfortable treatment can lead to fear and resistance to FSN needles, increasing the risk of injury and may even affect research data. Anesthesia may also affect the study results too. Hence, there is a need for strategies in FSN therapy on animals that minimize injury during the intervention. This study employs a chronic constriction injury model in Sprague-Dawley rats to induce neuropathic pain. This model replicates the pain induced by nerve injury observed in humans through surgical constriction of a peripheral nerve, mimicking the compression or entrapment seen in conditions such as nerve compression syndromes and peripheral neuropathies. We introduce an appropriate manipulation for easily inserting an FSN needle into the subcutaneous layer of the animal&#39;s body, including needle insertion and direction, needle retention, and swaying movement. Minimizing the rat&#39;s discomfort prevents the rat from being tense, which causes the muscle to contract and hinder the entry of the needle and improves the study efficiency.

저자 스포트라이트: FSN 치료의 치료 효과 발표 – 신경병증성 통증의 연구 및 임상 적용 연결

We present a protocol for using Fu's subcutaneous needling in a chronic constriction injury model to induce sciatic nerve pain in rats.

좌골 신경통에 대한 Fu의 피하 침의 효능: 만성 수축 손상 쥐 모델의 행동 및 전기 생리학적 변화

Fu&#39;s subcutaneous needling (FSN), an invented acupuncture technique from traditional Chinese medicine, is used worldwide for pain relief. However, the ...