살아있는 트리파노소마 브루세이(Trypanosoma brucei)의 동적 당염색체 pH 변화 측정

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08:50 min

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January 19th, 2024

DOI :

10.3791/66279-v

January 19th, 2024

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Daniel Call*¹, Sabrina S. Pizarro*²^,³, Erica Tovey¹, Emily Knight²^,³, Carrie Baumgardner²^,⁴, Kenneth A. Christensen¹, James C. Morris²

¹Department Chemistry and Biochemistry, Brigham Young University, ²Eukaryotic Pathogens Innovation Center, Clemson University, ³Department of Genetics and Biochemistry, Clemson University, ⁴Department of Physics and Astronomy, Clemson University

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0:00

Introduction

1:44

Sample Preparation for Flow Cytometry to Measure pHluorin2 Fluorescence in Living Trypanosoma brucei

2:29

Flow Cytometry Procedure to Measure Relative pH of the Glycosome in Living Trypanosoma brucei