In Vitro and In Vivo Approaches to Determine Intestinal Epithelial Cell Permeability

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25.2K Views

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10:22 min

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October 19th, 2018

DOI :

10.3791/57032-v

October 19th, 2018

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Ban-Ruo Li*¹^,², Jia Wu*¹^,², Hua-Shan Li¹^,², Zhi-Hui Jiang¹^,², Xiu-Min Zhou¹^,², Cai-Hua Xu¹^,², Ning Ding¹^,², Juan-Min Zha¹^,², Wei-Qi He¹^,²

¹Jiangsu Key Laboratory of Neuropsychiatric Diseases and Cambridge-Suda (CAM-SU) Genome Resource Center, Soochow University, ²Department of Oncology, The First Affiliated Hospital of Soochow University

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Keywords Intestinal Epithelial Cell Permeability

In Vitro

In Vivo

Inflammatory Bowel Disease

Gastrointestinal Diseases

Barrier Integrity

Caco 2bbe Cells

Transepithelial Electrical Resistance TER

Interferon Gamma

Rozdziały w tym wideo

0:04

Title

1:18

Plating and Maintenance of Caco-2bbe on Porous Polycarbonate Membranes

2:53

Use of Epithelial Meter for Measuring Transepithelial Electrical Resistance

4:39

Murine Model of Dextran Sulfate Sodium (DSS)-induced Colitis

6:07

Measuring the Epithelial Barrier Permeability in DSS-induced Colitis Mice

8:01

Results: Measure of Permeability of Intestinal Epithelial Cells

9:29

Conclusion

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