Plating and Maintenance of Caco-2bbe on Porous Polycarbonate Membranes
2:53
Use of Epithelial Meter for Measuring Transepithelial Electrical Resistance
4:39
Murine Model of Dextran Sulfate Sodium (DSS)-induced Colitis
6:07
Measuring the Epithelial Barrier Permeability in DSS-induced Colitis Mice
8:01
Results: Measure of Permeability of Intestinal Epithelial Cells
9:29
Conclusion
필기록
The overall goal of this experiment is to study the diseases caused by epithelial barrier dysfunction by measuring the intestinal epithelial cell permeability in vitro and in vivo. This method can help answer key questions in the intestinal barrie
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Two methods are presented here to determine intestinal barrier function. An epithelial meter (volt/ohm) is used for measurements of transepithelial electrical resistance of cultured epithelia directly in tissue culture wells. In mice, the FITC-dextran gavage method is used to determine the intestinal permeability in vivo.