Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

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6.4K Views

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10:28 min

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September 20th, 2018

DOI :

10.3791/58222-v

September 20th, 2018

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Anna M. Chiarella¹, Tiffany A. Wang², Kyle V. Butler³, Jian Jin³, Nathaniel A. Hathaway⁴

¹Chemical Biology and Medicinal Chemistry, Center for Integrative Chemical Biology and Drug Discovery, Curriculum in Genetics and Molecular Biology, University of North Carolina, ²College of Arts and Sciences, Chemical Biology and Medicinal Chemistry, Center for Integrative Chemical Biology and Drug Discovery, University of North Carolina, ³Chemical Biology and Drug Discovery, Pharmacological Sciences and Oncological Sciences, Icahn School of Medicine at Mount Sinai, ⁴Chemical Biology and Medicinal Chemistry, Center for Integrative Chemical Biology and Drug Discovery, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina

Podsumowanie

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Keywords Epigenetics

Gene Transcription

Chromatin Regulation

Chemical Epigenetic Modifiers

Gene Regulation Mechanisms

Cell Culture

293T HEK Cells

MESC

Polyethylenimine PEI Transfection

Viral Infection

Cell Passage

Trypsinization

Rozdziały w tym wideo

0:04

Title

0:49

Cell Line Culture for Lentivirus Production

2:27

Lentiviral Infection of Mouse Embryonic Stem Cells (mESCs)

5:28

Chemical Epigenetic Modifiers (CEM)s Preparation and Treatment

6:47

Microscopic and Flow Cytometry Analysis of Expression

8:55

Results: Effective Gene Repression Using Chemical Epigenetic Modifiers

10:00

Conclusion

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