Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
6.4K Views
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10:28 min
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September 20th, 2018
DOI :
September 20th, 2018
•1Chemical Biology and Medicinal Chemistry, Center for Integrative Chemical Biology and Drug Discovery, Curriculum in Genetics and Molecular Biology, University of North Carolina, 2College of Arts and Sciences, Chemical Biology and Medicinal Chemistry, Center for Integrative Chemical Biology and Drug Discovery, University of North Carolina, 3Chemical Biology and Drug Discovery, Pharmacological Sciences and Oncological Sciences, Icahn School of Medicine at Mount Sinai, 4Chemical Biology and Medicinal Chemistry, Center for Integrative Chemical Biology and Drug Discovery, Curriculum in Genetics and Molecular Biology, Lineberger Comprehensive Cancer Center, University of North Carolina
Chapters in this video
0:04
Title
0:49
Cell Line Culture for Lentivirus Production
2:27
Lentiviral Infection of Mouse Embryonic Stem Cells (mESCs)
5:28
Chemical Epigenetic Modifiers (CEM)s Preparation and Treatment
6:47
Microscopic and Flow Cytometry Analysis of Expression
8:55
Results: Effective Gene Repression Using Chemical Epigenetic Modifiers
10:00
Conclusion
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