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Preparation of Exosomes for siRNA Delivery to Cancer Cells

24.7K Views

09:59 min

December 5th, 2018

DOI :

10.3791/58814-v

December 5th, 2018


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Exosomes

Rozdziały w tym wideo

0:04

Title

0:51

Bioreactor Flask Cell Culture

1:52

Conditioned Medium (CM) Harvesting from the Bioreactor Flask

2:41

Exosome Isolation onto a Sucrose Cushion

5:02

siRNA Encapsulation into Exosomes by Electroporation

6:11

Removal of Free siRNA Using Size Exclusion Chromatography (SEC)

7:17

Results: Effective Intracellular Delivery of siRNA Using siRNA-encapsulated Exosomes

9:11

Conclusion

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