Iniciar sesión

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

8.1K Views

•

10:10 min

•

March 31st, 2019

DOI :

10.3791/59382-v

March 31st, 2019

•

8,094 Views

Huacheng Luo¹, Amin Sobh², Christopher D. Vulpe², Edmond Brewer¹, Sinisa Dovat¹, Yi Qiu³, Suming Huang¹

¹Department of Pediatrics, Pennsylvania State University College of Medicine, ²Department of Physiological Sciences, University of Florida, ³Department of Anatomy and Cell Biology, University of Florida

Capítulos

Transcribir

Resumen

Explorar más videos

CRISPR sgRNA Library

CTCF Boundaries

HOX Gene Regulation

Pooled SgRNA Screening

Lentiviral Transduction

MOLM13 Cells

Puromycin Selection

MOI Optimization

Noncoding Regulatory Elements

Now Playing

10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

8.1K Views

10:32

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

Videos relacionados

11.0K Views

06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Videos relacionados

5.7K Views

13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Videos relacionados

10.3K Views

12:24

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

Videos relacionados

9.2K Views

10:28

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Videos relacionados

8.5K Views

08:27

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Videos relacionados

10.7K Views

11:39

Mating-based Overexpression Library Screening in Yeast

Videos relacionados

7.5K Views

06:28

Screening Cotton Genotypes for Reniform Nematode Resistance

Videos relacionados

10.1K Views

06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Videos relacionados

7.2K Views