HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

0:49

The High Titer sgRNA Library Lentivirus Generation

1:44

Titration of Lentiviral Library in MOLM13 Leukemia Cells

2:59

Transduction of the Pooled CRISPR-Cas9 KO Library

4:20

Screening of the Pooled CRISPR-Cas9 KO Library with One-Step RT-qPCR

5:41

Verification of Integrated sgRNAs Positive Clones through Genotyping and Sanger Sequence

6:37

Detection of sgRNAs Induced Indel Mutation by Nuclease Digestion Assay

7:48

Results: Pooled CRISPR-Cas9 KO Library Screening and Identification

9:19

Conclusion