Short-Term Free-Floating Slice Cultures from the Adult Human Brain

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8.7K Views

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09:14 min

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November 5th, 2019

DOI :

10.3791/59845-v

November 5th, 2019

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Artur Fernandes¹^,², Niele Dias Mendes¹^,³, Glaucia Maria Almeida¹, Giovanna Orlovski Nogueira¹, Carla de Moraes Machado⁴, Jose de Anchieta de Castro Horta-Junior⁴, João Alberto Assirati Junior⁵, Norberto Garcia-Cairasco², Luciano Neder³, Adriano Sebollela¹

¹Department of Biochemistry and Immunology, Ribeirão Preto Medical School, University of São Paulo, ²Department of Physiology, Ribeirão Preto Medical School, University of São Paulo, ³Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School, University of São Paulo, ⁴Department of Anatomy, Institute of Biosciences, São Paulo State University, ⁵Clinical Hospital at the Ribeirão Preto Medical School, University of São Paulo

Podsumowanie

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Short term Cultures

Free floating Slice Cultures

Adult Human Brain

Brain Tissue

Resective Brain Surgery

Vibratome Specimen Disc

Section Thickness

Transport Apparatus

Rozdziały w tym wideo

0:04

Title

1:14

Setting Up the Slicing Apparatus

2:14

Sample Collection

2:45

Slicing

4:14

Culture

5:18

Morphological Evaluation

6:39

Results: Analysis of the Short-Term Free-Floating Slice Cultures

8:12

Conclusion

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