Results: Analysis of the Short-Term Free-Floating Slice Cultures
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We present a protocol to prepare free-floating slice cultures from brain tissue collected from living human donors submitted to resective brain surgery. This culture is amenable to perform biochemical and cell biology assays or immunohistochemistr
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A protocol to prepare free-floating slice cultures from adult human brain is presented. The protocol is a variation of the widely used slice culture method using membrane inserts. It is simple, cost-effective, and recommended for running short-term assays aimed to unravel mechanisms of neurodegeneration behind age-associated brain diseases.