Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases
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14.4K Views
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14:57 min
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October 10th, 2020
DOI :
October 10th, 2020
•1Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine, University of Geneva, 2Section of Biology, Faculty of Science, University of Geneva, 3Onco-Hematology Unit, Department of Women, Children-Adolescents, University Hospitals of Geneva
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Rozdziały w tym wideo
0:00
Introduction
0:56
Preparation of the Enzymatic Solution
3:10
Michaelis-Menten Constant of CDNB with GST
4:42
Absorbance of Potential GST Inhibitors
6:08
Determination of the IC50
7:49
Determination of the Type of Inhibition and Ki
9:46
Results: Testing the Potential of Curcumin as a GST Inhibitor
13:56
Conclusion
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