Spectrophotometric Screening for Potential Inhibitors of Cytosolic Glutathione S-Transferases

14.3K Views

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14:57 min

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October 10th, 2020

DOI :

10.3791/61347-v

October 10th, 2020

•

Shannon K. D. Robin¹^,², Marc Ansari*¹^,³, Chakradhara Rao S. Uppugunduri*¹^,³

¹Research platform of Pediatric Onco-Hematology, Department of Paediatrics, Gynaecology and Obstetrics, Faculty of Medicine, University of Geneva, ²Section of Biology, Faculty of Science, University of Geneva, ³Onco-Hematology Unit, Department of Women, Children-Adolescents, University Hospitals of Geneva

Summary

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Spectrophotometric Screening

Cytosolic Glutathione S transferases

Absorbance Measurement

Enzyme Activity

Protein Concentration

Chapters in this video

0:00

Introduction

0:56

Preparation of the Enzymatic Solution

3:10

Michaelis-Menten Constant of CDNB with GST

4:42

Absorbance of Potential GST Inhibitors

6:08

Determination of the IC50

7:49

Determination of the Type of Inhibition and Ki

9:46

Results: Testing the Potential of Curcumin as a GST Inhibitor

13:56

Conclusion

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