Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Please note that all translations are automatically generated. Click here for the English version.

3.8K Views

•

12:19 min

•

April 22nd, 2022

DOI :

10.3791/63089-v

April 22nd, 2022

•

Hui-Lan Chang*¹, Kang-Yi Su*¹^,², Steven D. Goodman³, Wern-Cherng Cheng², Liang-In Lin¹^,², Ya-Chien Yang¹^,², Sui-Yuan Chang¹^,², Woei-horng Fang¹^,²

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, ²Department of Laboratory Medicine, National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University

Podsumowanie

Przeglądaj więcej filmów

Uracil DNA Glycosylase

DNA Repair

MALDI TOF Mass Spectrometry

Enzyme Activity Assay

AP Product

Label free Method

E coli Uracil DNA Glycosylase

Rozdziały w tym wideo

0:04

Introduction

1:28

DNA Glycosylase Assay

4:43

Transferring UDG Reaction Products to a Matrix Chip

5:33

Setting up the Assay Parameters on the Mass Spectrometer

7:19

Mass Spectrometer Operation, Viewing Mass Spectra, and Analyzing the Data

9:03

Results: UDG Assay Using MALDI-TOF MS for AP Site Containing Product Analysis

11:01

Conclusion

Powiązane Filmy

09:18

Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics

9.8K Views

09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

13.7K Views

08:03

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

10.0K Views

10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

10.0K Views

14:02

Glycoproteomics of the Extracellular Matrix: A Method for Intact Glycopeptide Analysis Using Mass Spectrometry

12.2K Views

08:16

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

8.0K Views

11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

9.5K Views

11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

9.3K Views

07:31

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

5.6K Views

08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

7.2K Views