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Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

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12:19 min

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April 22nd, 2022

DOI :

10.3791/63089-v

April 22nd, 2022

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3,699 Views

Hui-Lan Chang*¹, Kang-Yi Su*¹^,², Steven D. Goodman³, Wern-Cherng Cheng², Liang-In Lin¹^,², Ya-Chien Yang¹^,², Sui-Yuan Chang¹^,², Woei-horng Fang¹^,²

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, ²Department of Laboratory Medicine, National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics, The Ohio State University

Chapitres

Transcription

Résumé

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Uracil DNA Glycosylase

MALDI TOF Mass Spectrometry

DNA Repair

AP Product

Label free

Enzyme Activity Assay

Guanine uracil Base

Reaction Buffer

Substrate Preparation

Reaction Termination

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