Human Liver Spheroids from Peripheral Blood for Liver Disease Studies
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09:51 min
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January 27th, 2023
DOI :
January 27th, 2023
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Rozdziały w tym wideo
0:05
Introduction
0:58
Preparation of Hepatocyte Differentiation Media
1:27
Culturing Hepatic Cells Differentiated from Blood-Derived Pluripotent Stem Cells (BD-PSCs)
2:14
3D Spheroid Hepatic Differentiation
3:34
Immunofluorescence Analysis of Newly Generated 2D Liver Cell Cultures
4:59
Live Staining of Newly Formed Liver Spheroids
6:01
Examination of Spheroids Using a Fluorescence Microscope
7:32
Results: Differentiation of Human BD-PSCs into Endoderm/Hepatic Progenitor Cells and Hepatocytes
9:11
Conclusion
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