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Human Liver Spheroids from Peripheral Blood for Liver Disease Studies

1.6K Views

09:51 min

January 27th, 2023

DOI :

10.3791/64703-v

January 27th, 2023


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Human Liver Spheroids

Rozdziały w tym wideo

0:05

Introduction

0:58

Preparation of Hepatocyte Differentiation Media

1:27

Culturing Hepatic Cells Differentiated from Blood-Derived Pluripotent Stem Cells (BD-PSCs)

2:14

3D Spheroid Hepatic Differentiation

3:34

Immunofluorescence Analysis of Newly Generated 2D Liver Cell Cultures

4:59

Live Staining of Newly Formed Liver Spheroids

6:01

Examination of Spheroids Using a Fluorescence Microscope

7:32

Results: Differentiation of Human BD-PSCs into Endoderm/Hepatic Progenitor Cells and Hepatocytes

9:11

Conclusion

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