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National Cheng Kung University

16 ARTICLES PUBLISHED IN JoVE

Education

A Fabrication Method for Highly Stretchable Conductors with Silver Nanowires

Chia-Wei Chang¹, Shih-Pin Chen¹, Ying-Chih Liao¹

¹Department of Chemical Engineering, National Taiwan University

A simple synthesis method is used to chemically solder silver nanowire thin film to fabricate highly stretchable and conductive metal conductors.

Immunology and Infection

Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic Poly(I:C)

Ke-Huan Chow^*1, Zihao Yan^*1,2, Wei-Li Wu¹

¹Division of Biology and Biological Engineering, California Institute of Technology, ²Harvard Medical School

Maternal immune activation (MIA) is a model for an environmental risk factor of autism and schizophrenia. The goal of this article is to provide a step-by-step procedure of how to induce MIA in the pregnant mice in order to enhance the reproducibility of this model.

Behavior

An Innovative Running Wheel-based Mechanism for Improved Rat Training Performance

Chi-Chun Chen¹, Chin-Lung Yang², Ching-Ping Chang^3,4

¹Department of Electronic Engineering, National Chin-Yi University of Technology, ²Department of Electrical Engineering, National Cheng Kung University, ³Department of Biotechnology, Southern Taiwan University of Science and Technology, ⁴Department of Medical Research, Chi Mei Medical Center

This study presents an innovative running wheel-based animal mobility system to quantify an effective exercise activity in rats. A rat-friendly testbed is built, using a predefined adaptive acceleration curve, and a high correlation between the effective exercise rate and the infarct volume suggests the protocol's potential for stroke prevention experiments.

Immunology and Infection

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System

Cheng-Ju Kuo^1,2, Sin-Tian Wang^1,2, Chang-Shi Chen^1,2

¹Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University

A detailed protocol of a mouse model for enterohemorrhagic E. coli (EHEC) colonization by using bioluminescence-labeled bacteria is presented. The detection of these bioluminescent bacteria by a non-invasive in vivo imaging system in live animals can advance our current understanding of EHEC colonization.

Cancer Research

The Establishment of a Lung Colonization Assay for Circulating Tumor Cell Visualization in Lung Tissues

Tsung-Cheng Lin¹, Ying-Chih Liao², Wen-Tsan Chang^1,2, Cheng-Han Yang¹, Li-Hsin Cheng¹, Megan Cheng³, Hung-Chi Cheng^1,2

¹The Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, ³Trauma Office, Children's National Health System

An animal model is needed to decipher the role of circulating tumor cells (CTCs) in promoting lung colonization during cancer metastasis. Here, we established and successfully performed an in vivo assay to specifically test the requirement of polymeric fibronectin (polyFN) assembly on CTCs for lung colonization.

Education

Uncoupling Coriolis Force and Rotating Buoyancy Effects on Full-Field Heat Transfer Properties of a Rotating Channel

Shyy Woei Chang¹, Wei-Ling Cai¹, Hong-Da Shen¹, Kuo-Ching Yu¹

¹Department of System and Naval Mechatronic Engineering, National Cheng Kung University

Here, we present an experimental method for decoupling the interdependent Coriolis-force and rotating-buoyancy effects on full-field heat transfer distributions of a rotating channel.

Immunology and Infection

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

Yi-Wei Chen¹, Wen-Chien Ko^1,2, Chang-Shi Chen^1,3, Po-Lin Chen^2,4

¹Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, ³Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, ⁴Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University

Here, we introduce three different experiments to study Aeromonas infection in C. elegans. Using these convenient methods, it is easy to evaluate the toxicity among and within Aeromonas species.

Biology

Primary Culture of Rat Adrenocortical Cells and Assays of Steroidogenic Functions

Yung-Chia Chen^1,2, Bu-Miin Huang³

¹Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, ²Department of Anatomy, School of Medicine, Kaohsiung Medical University, ³Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University

The hormone secreted by the adrenal cortex is vital for animals against stress and diseases. Here, we present a protocol to culture the primary rat adrenal cells. It could be a good in vitro platform for investigating the mechanisms of the reagent of interest in adrenal steroidogenesis and lipid biosynthesis.

Immunology and Infection

A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease

Ting-Jing Shen^1,2, Ming-Kai Jhan^1,2, Jo-Chi Kao^1,2, Min-Ru Ho^1,2, Tsung-Ting Tsai^1,2, Po-Chun Tseng^1,2, Yung-Ting Wang^1,2, Chiou-Feng Lin^1,2,3

¹Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, ²Department of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, ³Center of Infectious Disease and Signaling Research, National Cheng Kung University

Here, we present a protocol for creating an immunocompetent ICR (Institute of Cancer Research) murine model of central nervous system infection to display the development of neuropathy. Monitoring acute viral encephalitic disorders by identical disease scores could be performed for showing dengue virus-induced neuropathy in vivo.

Behavior

Measuring Biophysical and Psychological Stress Levels Following Visitation to Three Locations with Differing Levels of Nature

Yun Chang¹, Alan Ewert², Lisa M. Kamendulis³, Barbara A. Hocevar³

¹School of Kinesiology and Recreation, Illinois State University, ²Department of Recreation, Park, and Tourism Studies, Indiana University, ³Department pf Environmental and Occupational Health, School of Public Health-Bloomington, Indiana University

The purpose of this paper is to identify changes in stress levels after visitation to three different settings and to describe the methods used in identifying stress levels based on measures of salivary cortisol, α-amylase, and a psychological self-report instrument.

Immunology and Infection

Rose Bengal-Mediated Photodynamic Therapy to Inhibit Candida albicans

Jia-Horung Hung^*1,2, Zhao-Xiang Wang^*3,4, Yuan-Hsin Lo⁵, Chaw-Ning Lee^3,6, Yun Chang⁷, Rita Yuchiao Chang⁷, Chien-Chun Huang³, Tak-Wah Wong^3,8,9

The growing incidence of drug-resistant Candida albicans is a serious health issue worldwide. Antimicrobial photodynamic therapy (aPDT) may offer a strategy to fight against drug-resistant fungal infections. The present protocol describes Rose bengal-mediated aPDT efficacy on a multidrug-resistant C. albicans strain in vitro.

Medicine

A Mice Model of Chlorhexidine Gluconate-Induced Peritoneal Damage

Min-Yu Chang^*1,2, Hsi-Hao Wang^*1,2, Li-Hung Chen^*1, Jhen Gao^*1, Shih-Yuan Hung^1,2, Yuan-Yow Chiou^3,4, Yi-Che Lee^1,2,5

¹School of Medicine, College of Medicine, I-Shou University, ²Division of Nephrology, Department of Internal Medicine, E-DA Hospital, ³Department of Pediatrics, National Cheng Kung University Hospital, ⁴Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, ⁵Division of Nephrology, Department of Internal Medicine, E-DA Dachang Hospital

The present protocol establishes a peritoneal dialysis (PD) mouse model of chlorhexidine gluconate (CG)-induced peritoneal fibrosis. The current model is simple and easy to use compared to other PD animal models.

Neuroscience

Intracerebroventricular Delivery of Gut-Derived Microbial Metabolites in Freely Moving Mice

Chia-Wei Liou^*1,2, Tzu-Hsuan Yao^*2, Wei-Li Wu^1,2

¹Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Physiology, College of Medicine, National Cheng Kung University

Gut-derived microbial metabolites have multifaceted effects leading to complex behavior in animals. We aim to provide a step-by-step method to delineate the effects of gut-derived microbial metabolites in the brain via intracerebroventricular delivery via a guide cannula.

Biology

Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing

Szu-Ying Chen^1,2, Po-Hsien Huang^1,2

¹Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, ²Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University

This protocol provides an easy-to-follow workflow to conduct poly(A) RNA purification, bisulfite conversion, and library preparation using standardized equipment for a biological sample of interest.

Bioengineering

Finite Element Analysis Model for Assessing Expansion Patterns from Surgically Assisted Rapid Palatal Expansion

Jia-Hong Lin^*1, Guan-Lin Wu^*2, Chun-Kai Chiu², Steven Wang³, Chun-Hsi Chung¹, Chenshuang Li¹

¹Department of Orthodontics, School of Dental Medicine, University of Pennsylvania, ²Department of Biomedical Engineering, College of Engineering, National Cheng Kung University, ³Department of Oral and Maxillofacial Surgery/Pharmacology, School of Dental Medicine, University of Pennsylvania

A set of novel finite element models of surgically assisted rapid palatal expansion (SARPE) that could perform a clinically required amount of expander activation with various angles of buccal osteotomy was created for further analysis of the expansion patterns of the hemimaxillae in all three dimensions.

Biology

Induction of Petite Colonies in Candida glabrate via Rose Bengal-Mediated Photodynamic Therapy

Cai-Ying Yang^*1,2, Jia-Horung Hung^*3, Chi-Jung Wu^4,5, Zhao-Xiang Wang^1,2, Shih-Han Wang⁶, Hao-Chun Liaw², I-Huang Lin³, Chun-Keung Yu^1,6, Tak-Wah Wong^2,7,8

The significance of petite colonies in Candida spp. drug resistance has not been fully explored. Antimicrobial photodynamic therapy (aPDT) offers a promising strategy against drug-resistant fungal infections. This study demonstrates that rose bengal-mediated aPDT effectively deactivates Candida glabrata and induces petite colonies, presenting a unique procedure.