Name: contrast enhanced vessel imaging using microct
Uploaded: 2011-01-27T17:30:00
Description: Watch this Scientific Journal Video about Contrast Enhanced Vessel Imaging using MicroCT at JoVE.com

<p class="jove_content">This work was supported in part by NCI award 5R01CA133229-03.</p>

<p class="jove_title">1.    Animal Injections</p>
<p class="jove_content">Prior to intravenous tail vein injection, the tail skin should be cleaned with isopropyl alcohol scrubs for sterilization purposes. Mice should  then be injected with 0.2 mL/20 g of a 20-mg iodine/mL iodinated bloodpool contrast agent (eXIA 160XL; Binito Biomedical, Inc. Ottawa, ON, Canada) via the distal tail vein using low dead space &frac12; CC U-100 28G&frac12; Insulin Syringes (Product #329461, Becton Dickinson and company, NJ, USA).</p>
<p class="jove_title">2.    A...

<ol>
	<li>Holdsworth, D. W., Thornton, M. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Micro-CT+in+small+animal+and+specimen+imaging.">Micro-CT in small animal and specimen imaging.</a> <em style='font-style: italic'>Trends in Biotechnology</em>. <b>20</b>, 417-424 (2002).</li><li>Kindlmann, G. L., Weinstein, D. M., Jones, G. M., Johnson, C. R., Capecchi, M. R., Keller, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Practical+Vessel+Imaging+by+Computed+Tomography+in+Live+Transgenic+Mouse+Models+for+Human+Tumors.">Practical Vessel Imaging by Computed Tomography in Live Transgenic Mouse Models for Human Tumors.</a> <em style='font-style: italic'>Molecular Imaging</em>. <b>4</b>, 417-424 (2005).</li></ol>

<p class="jove_content">The foremost goal of this technique of contrast enhanced vessel imaging using microCT is to provide optimal method for generating high quality datasets for analysis of vascular networks in live mice. For microCT imaging, better signal-to-noise ratio of soft tissue is achieved by longer scan times with increased numbers of views and number of frames per view <sup>2</sup>.</p>
<p class="jove_content">Vessel identification is critically dependent upon accurate soft tissue identification and differentiation from bone. eXIA ...

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<td>eXIA 160XL</td>
<td>&nbsp;</td>
<td>Binito Biomedical, Inc.</td>
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contrast enhanced vessel imaging using microct

<p class="jove_content">Microscopic computed tomography (microCT) offers high-resolution volumetric imaging of the anatomy of living small animals. However, the contrast between different soft tissues and body fluids is inherently poor in micro-CT images <sup>1</sup>. Under these circumstances, visualization of blood vessels becomes a nearly impossible task. To overcome this and to improve the visualization of blood vessels exogenous contrast agents can be used. Herein, we present a methodology for visualizing the vascular network in a rodent model. By using a long-acting aqueous colloidal polydisperse iodinated blood-pool contrast agent, eXIA 160XL, we optimized image acquisition parameters and volume-rendering techniques for finding blood vessels in live animals. Our findings suggest that, to achieve a superior contrast between bone and soft tissue from vessel, multiple-frames (at least 5-8/ frames per view), and 360-720 views (for a full 360&deg; rotation) acquisitions were mandatory. We have also demonstrated the use of a two-dimensional transfer function (where voxel color and opacity was assigned in proportion to CT value and gradient magnitude), in visualizing the anatomy and highlighting the structure of interest, the blood vessel network. This promising work lays a foundation for the qualitative and quantitative assessment of anti-angiogenesis preclinical studies using transgenic or xenograft tumor-bearing mice.</p>

Watch this Scientific Journal Video about Contrast Enhanced Vessel Imaging using MicroCT at JoVE.com

Contrast Enhanced Vessel Imaging using MicroCT

<p class="jove_content">Contrast enhanced small animal vessel imaging by microCT is a rapid, cost-effective and high-throughput technique for serial <em>in situ</em> examination for tumor development, for analyzing the network of blood vessels that nourish them, and for following the response of tumors to preclinical therapeutic intervention(s).</p>

Microscopic computed tomography (microCT) offers high-resolution volumetric imaging of the anatomy of living small animals. However, the contrast between ...

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